Topic: v3.57U (preview) Read 7719 times

« **on:** April 09, 2022, 08:43:52 PM »

General Mitochondrial and Wellness Protocol
Author: Joshua Leisk, https://bornfree.life (V3.57U - 3rd-June-2023)
Based on: https://bornfree.life/understanding-the-model/6/updated-disease-model-wip/45/

[Download a printable version here]

This is an experimental protocol which may be helpful for supporting the unique nutritional and wellness / lifestyle requirements of people with:

Myalgic Encephalomyelitis / Chronic Fatigue Syndrome (ME/CFS).
Long COVID / Long Haulers. Viral / Post-Viral Chronic Fatigue. “Floxed”.
Migraines. Breathlessness. Hypoxia. Acidosis. Alkalosis / pH Dysregulation.
Dysregulated Cortisol. Dyslipidemia. Alopecia.
Small Fibre Peripheral Neuropathy (SFPN). Post Finasteride Syndrome (PFS).
Mast Cell Activation Syndrome (MCAS). Post SSRI Sexual Dysfunction (PSSD).
Diamine Oxidase (DAO) Deficiency and Elevated Histamine.
Brain Fog. Sleep disorders. Major and other types of Depression. Anxiety.
Postural Orthostatic Tachycardia Syndrome (POTS). Autism Spectrum Disorders (ASD).
Chronic Inflammatory Response Syndrome (CIRS). Fibromyalgia.
Dysautonomia. Frequent Urination. GI Disorders. Small Intestinal Bowel Overgrowth (SIBO).
B6 Toxicity. Candidiasis. Aspergillosis. Ehlers Danlos Syndrome (EDS). Joint Pain. Connective Tissue Disorders. Tinnitus. Polycystic Ovary Syndrome (PCOS). Erectile Dysfunction.
Elevated Cholesterols / Fasting Glucose / D-Dimer.

NB. While all efforts are continuing to be made in eliminating them, some temporary paradoxical effects may be observed during protocol commencement. As certain deficiencies are corrected and neurotransmitter homeostasis is reset, paradoxical effects may include: temporary adrenaline and heart rate increases. Resolving a severe copper deficiency may induce temporary kidney pain and nausea in some cases.

Herxheimer effects from microbial die-off can also be experienced. Existing "burning" sensations and headaches may also “flare”. Temporary alterations to libido may be expected. Improvements to adaptive immune function are expected to occur during this protocol, which may result in some short periods of productive, yet otherwise unpleasant tissue inflammation and/or fever, flu-like symptoms, nausea, fatigue, etc. Our Discord hosts a support group.

Monitoring Lactate Dehydrogenase (LDH) - target 100-150 mmol/L, D-dimer, cholesterols and fasting glucose via blood tests would be suggested.

Disclaimer: This protocol contains research / educational information ONLY. Always consult with your doctor to make sure this information is appropriate for you, especially if taking medications, eg. SNRI /SSRI, beta blockers, MAO inhibitors, drugs metabolised by CYP2D6, etc.

From v3.52 onwards, this protocol now includes a significant shift upstream in the cascade, targeting the pathogenic landscape and immune dysregulation.

INTRODUCTION
The human body contains a large number of elements. Many of these elements have been well-documented as having important biological activity in humans.

Our ongoing research has identified an IFN-α:IGN-γ immune dysregulation and persistent / cyclic inflammatory cascade that causes a functional insufficiency of at least 9 important elements that use a common pair of transporters - Divalent Metal Transporter 1 and Ferroportin. Further systemic deficiencies are observed in the metabolic cascade being described.

However, some variability exists in the pattern of insufficiencies. This can be due to alterations in renal function and general homeostasis by complex relationships between elements and some seemingly unrelated heavy metals. These complex relationships require further research.

The metabolic cascade appears in a surprising number of infections and chronic diseases. The model describes severe mitochondrial dysfunction, hypoxia, neurological alterations, collagen synthesis issues and immune deficiency. This is not an exhaustive list. The pattern of elemental deficiencies are also predictive of various disease features, when mapped to impairment of related enzymatic reactions. A preview of the disease model and biochemistry pathways being described in our upcoming paper have been made available on the website and Discord server.

Separate to the functional insufficiencies from inflammation, other testing methodology has shown a pattern of systemic mineral deficiencies which impair immune functions. Measuring, let alone correcting these nutritional deficiencies via diet and/or supplements can be extremely difficult due to the cyclic nature of the disease model and impaired absorption. Ongoing improvements to solving these problems are reflected in protocol updates.

LYMPHATIC MAINTENANCE REGIMEN

The lymphatic system is a network which spans the majority of the human body. Quite surprisingly, until very recently we were not aware that this also included the brain -
https://www.nih.gov/news-events/nih-research-matters/lymphatic-vessels-discovered-central-nervous-system

The lymphatic system has a number of important roles in immunity and circulation.

Lymphoid organs are the source of B and T lymphocytes. The system transports dietary fats and collects extracellular fluid and metabolites from tissues, returning them via lymphatic vessels to the bloodstream, preventing lymphoedema or catastrophic fluid buildup, pressure / pain and metabolic backlog.

A friendly primer on the lymphatic system can be found here - https://www.britannica.com/science/lymphatic-system.

The lymphatic system relies on breathing and muscle contractions to maintain healthy circulation and waste excretion.

In any disease where movement is being restricted, this creates additional difficulties for maintaining immune function, fat transport and metabolite circulation.

Manual therapy may compensate for this.

A regular session with a massage therapist who specialises in lymphatic drainage may help you assess and remediate any recurring lymphatic blockages. These may also be observed as being stiff, sore swollen tissues that can feature enlarged lymph nodes, such as in the neck, groin or armpits. A strong paradoxical effect may be felt the first time a blockage is cleared.
https://www.youtube.com/watch?v=dDrJajg2ZII
A daily regimen to maintain any problematic areas by hand or vibrating massage gun (used on a glancing angle), may also assist unlocking functional gains and preventing pain in chronic illnesses / infections. Conversely, not maintaining the lymphatic system may create a roadblock.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5954877/

LIVING WITHOUT CHRONIC YEAST, MOULD & FUNGAL INFECTIONS
Yeasts, moulds and fungi are found everywhere in nature and have an important role. They decompose animals, plants and other organisms, recycling their elements and other nutrients, thereby continuing the circle of life.

Usually these decomposing animals are not alive, however when an organism’s immune response is incapable of maintaining a defence against the normal daily background level of fungal challenge, the living organism will become food for yeast, mould and/or fungi. Various lifestyle influences contribute positively and negatively to maintaining this daily defence.

Lack of exposure to sunlight, lack of sufficient activity to maintain a specific immune function and/or lack of a protective microbiome can also contribute to an organism being more susceptible to mould. It has been previously said “we don’t grow old, we mould!”

The barrier function of the skin and mucosal layers play an important part in resilience against pathogens. Unfortunately, the human body has a number of tissues that are opportunistic for yeast / mould / fungal infections to thrive with minimal interference from direct immune activity.

Do you have a history of cracked lips / ears / toes / feet / rectum, flaking skin, dandruff, hair loss, red / inflamed skin patches / rashes, nasal inflammation, sleep breathing disorder, gut pain / issues, white or green/black film on tongue, discharge?

Orifices and tissues such as the nasopharynx, sinuses, throat, Eustachian tubes, ears, GI tract, rectum, vagina, feet, toes are areas which are not regularly exposed to direct sunlight. This list might well include all tissues, where someone is bed-bound or house-bound. These tissues provide “safe spaces” for any fungal spores to seed, colonise, create biofilms and thrive, without appropriate immune surveillance.

(Image source:https://commons.wikimedia.org/w/index.php?curid=30850944,)

(Image source:https://commons.wikimedia.org/w/index.php?curid=30850945)

Subgrouping for ME/CFS patients may have a relationship with the locations of infected tissues. In the case of a GI tract infection, this may also affect dietary absorption efficiency. The nasopharynx (and surrounding areas) may be very important tissues for chronic innate immune activation. A healthy microbiome appears to play a very important role in providing protection against invasive bacterial, yeast, mould and fungal infections.

Apart from generating gaseous toxins, ethanol and acetaldehyde - depleting silicon and other metals in compensation, many yeasts / mould / fungi and bacteria can cause dramatic metabolic alterations. They have an ability to break through our skin / epithelial tissue / mucosal barrier function from a relatively “safe space” and cause recurring / chronic immune activation during the invasion. This immune activation inhibits specific mitochondrial metabolism and reconfigures a number of other metabolic pathways toward combating these pathogens. However, while ultimately beneficial, these alterations can be quite debilitating, under certain conditions.

Additionally, whenever our immune system is successful in killing these microorganisms, their death can cause the circulation of other toxins which contribute to a well-known “die-off effect” occurring.

This die-off effect is responsible for a number of highly debilitating symptoms, such as headaches, nausea, additional fatigue, dizziness, swollen glands, bloating / gas, constipation or diarrhoea, joint or muscle pain, tachycardia, chills, cold hands / feet, itchiness, rashes, sweating and/or fever which resembles the “disulfiram effect”.

https://www.thecandidadiet.com/candida-die-off-symptoms/

This can cause an ongoing / recurring cycle of extreme unpleasantness, if these infections and their original “safe spaces” are not fully resolved and the layer of protective microbiome restored.

We have been actively researching a combination of some existing strategies to help kill these microorganisms and minimise the die-off symptoms. Some additional challenges relate to the lifecycle stages and biofilms being created, which provide further protection against our immune activities.

In addition to the supplement schedule included in this protocol, a more rapid clearance of any (somewhat) accessible microorganisms may be achieved by direct or topical interventions for these infected tissues. Commercial preparations are available with various levels of efficacy.

Popular antifungal soaps, shampoos and creams containing zinc pyrithione can be effective at inhibiting fungal overgrowth. However, these products are now banned in Europe due to concerns around causing DNA damage.

However, a successful intervention for these tissues may include first administering a solution to break up any protective biofilms, leaving this solution to incubate for 20-30 minutes before (where possible / appropriate) wiping the area clean and then administering a second solution to selectively inhibit these foreign cells. These 2 solutions can also be applied simultaneously. The process may be repeated daily for 1-2 weeks, or as necessary.

Ongoing testing and literature review has suggested that a solution of NAC and salt water alone can reliably break up many biofilms and has other beneficial functions. This exposes microbes to immune surveillance and interventions you may explore.

Further research has also suggested that a solution containing diluted tea tree oil, clove oil and other oils, when applied with or immediately following NAC yields a tolerable, yet significant inhibitory effect on bacteria, yeasts, moulds and fungi which may rival or exceed existing pharmacological interventions and is appropriate for a range of applications.

Commercial preparations made from tea tree oil and clove oil, are readily available as shampoos, vaginal douches, topical sprays, mouthwashes, creams and suppositories. https://iherb.com/pr/tea-tree-therapy-suppositories-with-tea-tree-oil-for-vaginal-hygiene-6-suppositories/14691

Available from your local chemist, Neilmed make sinus washing products - such as “Sinugator” and “Sinus Rinse”, which can be highly effective at pre-rinsing these difficult-to-reach mucosal tissues, 20 minutes prior to application of any antiseptic products.

1g Xylitol and/or eg. 2mg of liquid iodine can also be added to the Neilmed solution, for additional potency, however this may not be appropriate for daily use. A temporary smell of iodine and some “burning” / “stinging” would also be expected where infected tissues exist.

DIY Antiseptic Nasopharynx Clean and Flush, Dental Rinse, Topical Spray Recipe:

Part 1:
Mix 500mg of NAC powder (preferably not from a capsule, as these usually have fillers, although these could also be filtered / strained after stirring), 500mg of sodium bicarbonate (baking soda), 1g of xylitol and >5 ml of water.

Part 2:
Separately, mix essential oils - 20 drops of tea tree oil, 80 drops of black seed oil, 6 drops of oregano oil, 6 drops of clove oil
(You can add more black seed oil if you find this too strong.)

Mix both parts together to form your final solution. Store in an empty glass bottle with a dropper for convenient dosing. Shake well before use.

To test this as a nasal rinse - while laying on your back, with your head tilted back and breathing through your mouth, drip about 6 drops of the solution into each nostril.

Let the solution run into the nasopharynx and incubate for a minute or so, while you “suffer” through some shockingly unpleasant “burning” for a few minutes (the first time), assuming you have infected tissue. (See the experiences reported in the Discord server for more information.)

Healthy tissues won’t be irritated, so if it burns - repeat this process daily until clear - it will get progressively much easier. Rotating your head slowly to each side and “equalising” with fluid in the nasopharynx may allow the solution access to the Eustachian tubes. Eventually blow / purge your nose, leaving the residue behind. A followup nasal probiotic may be very helpful.

To test this as a topical antiseptic - simply clean the area and then spray / apply the solution. A cotton tip could assist application to the ear canal or rectal administration.

To test this as a dental rinse - brush / floss as usual, then apply a few drops to your tongue and gums, or more to gargle and spit. NB. Unpleasant flavour. Brushing immediately afterwards may damage tooth enamel, unless the solution is pH balanced with eg. 1:1 sodium bicarbonate:NAC.

NB. As an antiseptic, small amounts reaching your throat isn’t likely to cause toxicity, whereas drinking it intentionally could be harmful. Also, using this antiseptic recipe while not using the other supplements from Stage 1 may be unnecessarily unpleasant and exacerbate symptoms.

A more gentle “ramp up”, prior to utilising this antiseptic recipe, could be to first use the Sinugator or Sinus Rinse products in the manner as described above, for up to a week beforehand. Your pharmacist can advise if numbing the area with lidocaine or other analgesics may also be appropriate, in some circumstances.
For more sensitive infected tissues, eg. ocular and vaginal infections, a pH balanced rinse / douche can be effective if used for a 1-2 week period. Seek medical guidance. Various commercial preparations are available. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1705857/
https://ph.betadine.global/en/ph/feminine-care/products/medicated-feminine-hygiene/betadine-10-vaginal-douche
https://www.frezyderm.us/intimate-area-care/intravaginal-care/intim-vaginal-douche-soda-ph-9/

Per 240mL of boiled / cooled water, mix 5g of sodium bicarbonate (baking soda) with 5g NAC. This allows an acid:base reaction (and gas release) to occur, which helps create a pH balanced solution. This can be combined with 2 drops of 5% Lugol’s iodine and/or 5g xylitol.
Eyebath - use eg. 30mL of the solution to bathe each eyeball, also cleaning the eyelids, lashes.
Male urethra - administer eg. 5-30mL of the solution via a (needleless) syringe to the opening.
Vagina - administer eg. 120mL, using a suitable douche tool and with elevated legs.
Lungs - administer eg. 5mL via a nebuliser. (https://www.amazon.com.au/dp/B084W812C9)

Protective Probiotics
Following on from any efforts in clearing unwanted biofilms and microbial species overgrowth in the eyes, sinuses, nasopharynx, mouth, throat, oesophagus, lungs, GI tract, vagina, urethra and other mucosal tissues, further restorative efforts are usually needed in repopulating these tissues with helpful species to promote and maintain a healthy microbiome. Links to these products can be found in the “STEP 2 ORDER YOUR SUPPLEMENTS” section.

These helpful species assist in inhibiting unwanted species and preventing opportunistic growth. They can also provide helpful functions, such as metabolism of oxalates, histamine and production of short chain fatty acids and other compounds that are beneficial to humans.

Many of these species were traditionally found in the bacillus, lactobacillus and bifidobacterium genus, although recent taxonomy changes have relabelled / reclassified some of them, providing some minor confusion when researching species.

https://en.wikipedia.org/wiki/Bacillus
https://en.wikipedia.org/wiki/Bifidobacterium
https://en.wikipedia.org/wiki/Lactobacillus

Studies and dosing information for these microorganisms in different mucosal tissues can be found on the BornFree Discord server and/or the literature. This protocol currently includes a number of key probiotics relating to these studies and their described functions / benefits:

Youtheory Spore: B. coagulans, clausi, subtlis
Mood Super Strains Probiotic: L. rhamnosus, B. longum, bifidum, lactis, breve, infantis
Biogaia: L. reuteri
(Various sources, Kimchi): L. sakei (also used in food production to prevent spoilage, etc)

These can be directly administered to mucosal tissues, using appropriate methods - generally mixing a suitable amount of the probiotic (remove capsule where appropriate) with a small amount of clean water and then applying it where needed. eg.
Nostrils - cotton tip / fingertip and apply inside the nostrils.
Eustachian tubes - place drops of liquid into the nose and use the “equalisation” technique.
Mouth / throat / oesophagus / upper GI - gargle and swallow.
Lower GI tract - leave in capsule and swallow.
Vagina - add to a vaginal douche.
Eyes - place drops of liquid into the corners of the eyes and / or under the eyelids.

Please note these probiotics are included for specific functions and to generally promote a healthier balance in the microbiome. They are not intended to replace “GI-MAP” or similar stool tests which aim to identify microbiome imbalances / deficiencies. Although these types of stool tests are not always accurate or useful in identifying upper GI pathogens / overgrowth, they can still be very helpful in restoring a functional microbiome and may appear in future protocol updates, once a streamlined process is created.

Environmental Toxicity, Transference and Reinfection Cycle

When a person is chronically ill and confined to their bed / house, the transfer of pathogenic microbes from their body to their surroundings becomes a significant concern. Pathogenic microbes can transfer easily from the body to the environment and back again, causing an ongoing challenge to an already compromised immune system.

One of the primary concerns is the transfer of pathogenic microbes to the bed, pillows, sheets, and mattress. These surfaces can harbour pathogens and continue a cycle of infection.

Bed linen - sheets, pillowcases, and blankets, should be changed frequently and washed in hot water, with a hygienic detergent - to kill any pathogens present and arrest the cycle of reinfection.

Mattresses and pillows should also be rotated and disinfected regularly. A waterproof mattress protector can also help prevent the accumulation of moisture and bacteria. These will also need regular cleaning.

Another concern is the transfer of airborne pathogens through air conditioning systems. Air conditioning systems can spread pathogens throughout a room, increasing the risk of infection. It is important to clean / change the air filter frequently, and consider using an air purifier to help remove pathogens from the air.

Carpets, window frames, walls, ceilings, etc should also be inspected and maintained appropriately. Commercial air quality testing and mould remediation services are also available.

If not completely bed-bound and where the room doesn't have plants and/or pets (which can be excluded from the room for an hour), a relatively cheap ozone generator can be used to disinfect entire rooms, very effectively. Sunlight is also helpful in inhibiting certain microorganisms.

Overall, it is essential to maintain good hygiene practices - regularly disinfecting surfaces and fabrics, to prevent ongoing reinfection and unnecessary delays in progress.

Gastrointestinal Biofilms, Pathogens and Remediation
This protocol currently includes a number of staged interventions to address biofilms and pathogens in different locations inside the GI tract, such as Candex, Biofilm Phase 2 Advanced, NAC, spirulina, curcumin, prebiotics, targeted probiotics and water-fasting, with an aim of not unduly circulating endotoxins / acetaldehyde in the process of eliminating them, if at all possible. These strategies have been testing well and we are always exploring ways to improve on this process (please join the Discord community - https://discord.gg/CAMXV78).

One of the key challenges is that invasive microorganisms which breach the (mucosal) barrier function can trigger an IFN-gamma response, which causes collateral damage and inflammation to infected tissues in the process of oxidising pathogens, using reactive oxygen species.

If the rate of collateral oxidative damage + pathogenic damage exceeds the regenerative capacity of the tissue, this could further compromise the barrier function and dysregulate IFN-gamma activity, leading to a “deadlocked” cycle / state. External indicators for insufficient collagen synthesis rates may include hEDS symptoms, poor skin texture, chronic cranial instability, slipped rib syndrome, thoracic outlet syndrome, etc.

Co-infection of flesh-eating parasites such as worms, flukes, etc., can temporarily induce severe ME/CFS symptoms by directly attacking the epithelial layer and allowing opportunistic invasion by any/all microbial species.

If you suspect you have a parasitic infection, OTC pharmaceutical interventions such as mebendazole, albendazole and pyrantel could be appropriate. These are commonly taken as either a single dose intervention by all inhabitants of a household or multiple times over 2 weeks, per your pharmacist’s or doctor’s advice.

Additionally, various herbal products based on black walnut, cloves and wormwood have also shown as effective. eg. https://www.australianhealthfoods.com/product/ppc-herbs-herbal-tri-plex

There are some additional GI strategies being tested which may feature in future updates.

Xylitol:
5g of xylitol before meals to prevent acetaldehyde production, created when your food also feeds microorganisms. Can cause initial die-off effects.

Silicol Gel, Enterosgel:
Both of these readily-available products are non-absorbing silica-based gels that act as “sponges” for endotoxins / LPS / acetaldehyde and salts of heavy metals, backed by clinical trials. It is not yet known how much of an impact on “normal” mineral status this has.

Salt / Iodine-Starch Complex “Overgrowth Flush / Trojan Horse”:
Dissolve ½ teaspoon of quality sea salt into ½ cup of boiling water.
Slowly stir in 6 teaspoons of sifted potato starch, to prevent clumping.
Add ¼ dropper of 5% Lugol’s iodine (12.5mg, if using a different liquid iodine source or concentration), while stirring.
The solution should change colour to dark blue.
Add cool water until the glass is full.

Iodine binds to the starch and can transit the GI tract. The flush solution would be taken on an empty stomach. Consume 500ml of water, 30 minutes later. Expect unpleasant die-off symptoms / herxheimer-style reactions, diarrhoea, nausea. This flush could be performed for up to a week in the beginning of the protocol or during any water fasting stage, if overgrowth exists. A course of protective probiotics would follow this type of intervention process to prevent regrowth of unhelpful species.

Chlorella:
Chlorella is currently being tested as a more potent sIgA promoter and could be included as a follow-on after Aloe Vera has been used at full doses and no longer induces any herxheimer symptoms. Doses of Chlorella would range from 500mg to 1500mg.

THE IMPORTANCE OF “PACING” OR “JUST ENOUGH ACTIVITY”
Anyone who has experience with ME/CFS is likely to have gained a significant appreciation for the symptoms associated with “crashing” and Post Exertional Malaise (PEM).

A daily fear that too much activity will “crash” you and leave you with fatigue / malaise, flu-like symptoms and often tinnitus. A further fear that this may persist for days / weeks / months.

A hypothesis surrounding the mechanisms involved in PEM is being described in the upcoming paper, however the WIP diagrams are available on the website and Discord server.

Unfortunately, due to the trauma surrounding PEM, some people choose to severely limit their activity. This unfortunately has an impact on the capabilities of their immune system to deal with chronic infection.

One of the primary tools the innate immune system can use against microbial challenges is creating reactive oxygen species (ROS) to oxidise these pathogens. Interferon-gamma inhibits Complex I, which means that NADH generated by the TCA cycle can be diverted towards elevating NADPH, also powering NADPH oxidase and nitric oxide synthase, which then (like xanthine oxidase, which uses NAD+ instead), create reactive oxygen species to oxidise pathogens, damaging your cells in the process. This can also lead to fungal die-off symptoms.

Excess NADPH / low NADP with elevated NADH / low NAD+ can be observed / inferred by elevated cholesterols, impaired glucose metabolism, elevated cortisol / inverted diurnal release profile, low aldosterone, low testosterone, low BH4, impaired methylation and various other compensations.

For optimal immune response and hormones, “pacing” should always be attempted - testing the appropriate upper threshold for activity, each day, unless that threshold has been exceeded already. Over sufficient time, inactivity leads to “rotting”, which is wholly undesirable, as the immune response is expected to be insufficient for maintaining resilience against pathogens.

The combination of supplements here should help improve the exertion threshold and buffer against “crashing”. However, you may have already noticed that fungal “die-off” symptoms share some common features with PEM and crashing, as does any immune activity. This will add some challenges for identifying your upper limits for exertion.

NB. The upper limit for exertion will be artificially reduced when interferon-gamma is elevated and Complex I is inhibited, during any intense immune response. Increasing temperature elevates interferon-gamma levels potentially 10x. Spirulina and curcumin modify this pathway, favourably.

Proceed carefully, however the age-old phrase “no pain, no gain” absolutely applies here.

SLIPPED RIB SYNDROME, COSTOCHONDRITIS AND CHEST PAIN
The lower traps and rhomboids are essential muscles responsible for scapular control and stability. Weakness in these muscles can lead to poor scapular control, which can result in chronically pronated shoulders and thoracic outlet syndrome (TOS).

Pronated shoulders occur when the shoulder blades rotate forward, leading to a hunched posture. This can occur when the lower traps and rhomboids are weak and unable to maintain proper scapular alignment. The resulting forward shoulder position can cause tension and compression in the neck and shoulder region, leading to TOS.

TOS is a condition where the nerves, lymphatic thoracic duct and blood vessels that pass through the thoracic outlet become compressed, leading to pain, numbness, and weakness in the arm and hand. Forward head position will further contribute to the progression of this syndrome. This can cause impaired immune response and water retention / lymphoedema.

To prevent TOS and other related conditions, it is essential to maintain proper scapular control and stability. This can be achieved through exercises that strengthen the lower traps and rhomboids, such as scapular retraction and shoulder blade squeezes. Additionally, maintaining good posture and avoiding activities that require prolonged shoulder and arm elevation can help prevent TOS, however when this position occurs during sleep, it can be hard to avoid.

Slipped rib syndrome, costochondritis, and worrying chest pain can all be downstream effects of a poorly seated rib - usually between T6-T8. When a rib is not properly aligned, it can cause thoracic instability, leading to overworked intercostal muscles and inflammation in the surrounding tissues. This is a common issue where hEDS metabolism is present. hEDS metabolism is described in the disease model as a "normal feature" whenever innate immune response pathways are activated and/or specific mineral deficiencies are present.

Slipped rib syndrome occurs when a rib slips out of place and moves too easily, causing pain and discomfort. Costochondritis is inflammation of the cartilage that connects the ribs to the breastbone, which can also cause significant chest pain, which can mimic having a cardiac event.

When a rib is poorly seated, it can create an unstable environment for the intercostal muscles that are responsible for breathing. With mitochondrial dysfunction, these overworked muscles can become fatigued and inflamed, leading to scary chest pain and difficulty breathing.

It is wise to seek medical attention if you experience chest pain, as it can be a symptom of more severe conditions such as a heart attack or pulmonary embolism. However, if you have chronic chest pain that is related to slipped rib syndrome or costochondritis, treatment may include correcting the alignment of the rib and regular physical therapy, once any hEDS metabolism is corrected. Like any other resistance training, this tissue rehabilitation process will normally take weeks or months to complete. A (free) posture rehabilitation course can be found in the Discord.

CORTISOL, LIMBIC SYSTEM, GLYCOGEN AND INTERFERON-GAMMA
Cortisol is the “master” negative regulator for IFN-gamma.

What this means is that any/all influences which promote cortisol also decrease IFN-gamma activity and provide some “relief” for most of the symptoms observed in ME/CFS. This allows periods of increased functionality, while simultaneously inhibiting the necessary suppression of the pathogens that are triggering the innate immune response pathways.

In the disease model, we have described how cortisol acts as a “sensor” and signalling relay for insufficiencies of NAD+, P5P and glucose / glycogen, via phosphatidylserine and 11HSDβ1/2 flux. The metabolism for NAD+ and P5P is altered by IFN-gamma and they act as upstream sensors for specific IFN-gamma activities which can upset the metabolism or cause excessive tissue damage via oxidative stress. An inverted diurnal cortisol release pattern is expected.

When any of the sensed metabolites are insufficient, cortisol increases, signalling for an increase of epinephrine and catabolic activity, plus inhibition of IFN-gamma. This allows metabolites to replenish and partially restores normal metabolism.

As the metabolites reach sufficiency, cortisol levels decrease. If any triggers for IFN-gamma are still present, then IFN-gamma activity will resume and the cycle will repeat, as necessary.

Exercise and heat can increase IFN-gamma. Dietary supplements can support IFN-gamma activity. Cortisone medications are potent immunosuppressants and will inhibit IFN-gamma.

Appropriate carbohydrate intake and metabolism supports glycogen. Nicotinamide + ribose can support NAD+ biosynthesis. Creatine can reduce the “costs” associated with use of this pathway and increase glucose transport. Magnesium and zinc can support B6->P5P metabolism.

Hypothyroidism affects both glucose metabolism and “B6 toxicity” / P5P insufficiency. The thyroid hormone, T3 (tyrosine, iodide, selenium, calcium) is required to maintain FMN levels, needed for P5P recycling and to induce pyruvate flux into the TCA cycle.

The primary sex hormones (testosterone / DHT in males, estradiol in females) sense the elevation of NADPH (created during physical / metabolic activity), with sufficiency of NAD+, magnesium and zinc. These primary sex hormones then promote creatine synthesis and glucose uptake. They inhibit cortisol, promote nitrogen metabolite recycling and glycogen synthesis. This also allows IFN-gamma activity to increase, which is also needed for tissue adaptations to exercise. Non-optimal sex hormones can cause chronic cortisol increase.

Importantly, lifestyle factors and learned responses to stimulus - namely stress / fear / anxiety cause the limbic system to promote cortisol levels and inhibit the IFN-gamma pathway / immune response, while decreasing glycogen stores. As this also decreases “ME/CFS” symptoms, a persistent cycle of fear / anxiety and immune suppression can also be learned / imprinted.

STEP 1 - COLLECT YOUR DATA
a) Research suggests that a Hair Toxin Mineral Analysis (HTMA) may provide an approximation of your FUNCTIONAL mineral status, averaged over the period of follicle growth, sampled downstream of the transporter alterations from inflammation - where circulating minerals can be sequestered inside various brain, liver and kidney cells and consequently low in other cells. A list of vendors offering a compatible list of markers and reference ranges is provided here:

Doctors Data and other compatible laboratories:
AU - https://www.toxno.com.au/articles/heavy-metals/hair-tissue-mineral-analysis-hma-or-htma-in-australia/ (can manually add rubidium)
EU / UK - https://regeneruslabs.com/products/hair-toxic-essential-elements-1
EU - https://www.biocoherence.eu/en/shop/htma/ (doesn't include rubidium)
EU - https://www.lifelinediag.eu/en/product/eha-standard-en/#products (doesn't include rubidium)
NZ - https://www.houseofhealth.co.nz/product/hair-toxic-mineral-analysis/
PL - https://analizawlosa.com.pl/produkt/analiza-wlosa/
UK, some EU - https://www.lauristonlabs.co.uk/product/hair-elements-doctors-data/
US - https://www.walkinlab.com/products/view/dd-hair-elements-profile-doctors-data (Not available in NY/ NYC and other areas.)

Hair collection guide:
1. Please ignore any conflicting vendor-specific instructions.
2. The hair needs to be clean, well-rinsed, dry, untreated and uncoloured. Unwashed hair may provide false (elevated) data for sodium and potassium, from dried sweat. Use gloves.
3. Select suitable areas of sideburn, scalp and/or neck hair. If the hair is longer than 2.5cm / 1", first trim the sample area to this maximum length with clean stainless steel scissors. By using shorter hair, the report data relates to a more recent period of time.
4. Cut the (remaining) hair to be sampled as close to the skin as possible. Do not use clippers or an electric razor - metal contamination from tungsten blades, etc may occur. Collect at least 2 heaped tablespoons worth of hair, or approximately 250 mg or the sample may be rejected.
5. Place the hair sample in the sample kit envelope provided, or in a clean, clearly labelled envelope or sachet. Whilst Trace Elements Inc says not to use ziplock bags as apparently hair “sweats” and the sample may be rejected, Doctors Data supplies ziplock bags in their kit.

The results obtained from the HTMA data may be used to infer averaged intracellular mineral availability, downstream of any chronic inflammation, for the period of time in which the hair follicles grew. This is especially relevant for the 9 elements discussed in the introduction.

b) Research suggests that an Oligoscan report can provide an approximation of your ACTUAL mineral status, as inferred by the mineral content of the skin in your hand. This is an in-clinic test, so a local practitioner will be required. Your blood type, age, height and weight affects the sample calibration used to generate this report, so this information will be requested when you visit a clinic. Any inaccuracies can alter your results.

Unfortunately, a comprehensive list of providers is not yet available. An outdated list can be found here: https://annuaire.myoligoscan.com/. If you have a good (or bad) experience with an Oligoscan practitioner, please share details with our community Discord server. You can also manually enquire about local practitioners here: https://www.theoligoscan.com/nearest-provider

The set of elemental markers collected is almost the same as the Doctors Data HTMA, however it also includes silicon, fluorine and unfortunately does not include rubidium or strontium markers. These are not known to be directly affected by inflammation, meaning the HTMA data can be referenced to help supplementation planning. However, the Oligoscan report provides the most actionable set of objective markers to target when choosing mineral supplements, eg.

While not happy with the level of published evidence currently supporting this emerging technology, our testing showed a clear pattern of deficiencies in ME/CFS, Long Covid and C19 vaccine injured people, with minor variability, which was not observed in controls. Follow up Oligoscans match eg. supplements taken and previous data patterns.

c) A Great Plains Organic Acids Test (OAT) is a comprehensive report on urine metabolites.
It is available through local vendors or potentially, this website - https://gpldirect-international.com/collections/popular-test/products/organic-acids-test-oat

In addition to allowing interpretation of metabolic impairment from the patterns observed in these markers, page 1 of the report shows various markers for microbial interference. The remaining pages show the metabolic impacts for infection and malnutrition. Elevation of yeast, bacterial and fungal metabolites in OAT results have been seen consistently in our collected data, with matching symptoms. A further MycoTOX test may provide further clarity on species, if required.

A separate guide to interpreting these results is being drafted. Example interpretations of these reports can be found on the Discord server.

STEP 2 - ORDER YOUR SUPPLEMENTS
While the Oligoscan provides instant results, it will typically take 2-3 weeks to get your HTMA and OAT results.. and a potentially similar timeframe to order / receive supplements from any overseas vendors. Now would be an opportune time to order any/all supplements which do not require personalisation and each of the mineral supplements which are used early in the schedule.

Some products are not available domestically in all regions and will need to be imported from the US. As such, the “master list” is based around US suppliers. However, a list of local vendors / supplements for regions has been provided for some products. It is expected that local lists will expand over time. (PRODUCTS RECENTLY UPDATED are marked in GREEN)

[EU / Europe - alternate / domestic sources for some products]
Standard supplements, regardless of mineral data:
L. Reuteri - https://www.24evexia.com/BIOGAIA-PROTECTIS-FOR-GUT-COMFORT-30-CHEWABLE-TABLETS
NAC - https://www.amazon.de/N-Acetyl-L-Cysteine-Powder-Sulphur-Containing-Fermentation-Additives/dp/B07D3722WS
Reishi - https://www.lifeextensioneurope.de/reishi-extract-mushroom-complex
Lions Mane - https://www.amazon.de/dp/B07W668CZF
PQQ + CoQ10 - https://www.sunday.de/en/polyphenol-21-komplex.html
Xylitol - https://www.amazon.de/dp/B01FDHRONA
Biofilm Breaker (BP2A) - https://naturesfix.co.uk/product/biofilm-phase-2-advanced-60-vegcaps-priority-one-vitamins/
L. sakei (UK, EU) - https://kimchipower.co.uk/

Additional elements, as needed:
Calcium + Phosphorus - https://www.sunday.de/en/natural-calcium-400mg-high-dose-capsules-set.html
Potassium - https://www.amazon.de/-/en/Potassium-Tablets-Vegan-Additives-Dosage/dp/B08VRPR39L/
Iodine - https://www.amazon.de/-/en/Lugolsche-Solution-Pharmaceutically-according-Developed/dp/B07L87KPTB/
Monosodium Phosphate - https://atomscientific.com/product/mono-sodium-phosphate-anhydrous-food-grade

Foods, salts:
Sodium Chloride - https://www.amazon.de/-/en/dp/B07PBJG722
Potassium Chloride - https://www.amazon.de/-/en/No-Salt-Sodium-Free-Alternative/dp/B000H185N6/
Oat Bran - https://www.amazon.de/-/en/764087/dp/B06WVLBX5P
Ginger - https://www.amazon.de/Ginger-Powder-120-Additives-Laboratory/dp/B09B2GRNG7

[AU / Australia - alternate / domestic sources for some products]
Standard supplements, regardless of mineral data:
NAC - https://www.ebay.com.au/itm/204114762944
PQQ + CoQ10 - https://return2health.com.au/jarrow-qh-pqq-ubiquinol-pyrroloquinoline-quinone
Vimergy B12 - https://floweroflife.com.au/shop/vimergy-b12/
Psyllium Husk - https://www.woolworths.com.au/shop/productdetails/479953/macro-organic-psyllium-husk
Youtheory Spore - https://vitamingrocer.com.au/products/youtheory-spore-probiotic-171108
LGG Probiotic - https://www.chemistwarehouse.com.au/buy/81560/inner-health-on-the-go-60-capsules
L. Reuteri - https://blackshawspharmacy.com.au/products/bio-practica-biogaia-protectis-100-chewable-tablets
Creatine - https://www.chemistwarehouse.com.au/buy/74336/inc-creatine-500g
Xylitol - https://www.nutgrocer.com.au/xylitol-sweetener/
Biofilm Breaker (BP2A) - https://naturesfix.co.uk/product/biofilm-phase-2-advanced-60-vegcaps-priority-one-vitamins/
L. sakei (only available as a CHR-Hansen food-grade product via industry sources)
(AU) - https://www.mblsa.com.au/327850/MEAT-CULTURE-SAFE-PRO-B_dash_2-%28100gm_or_200kg%29/pd.php
(NZ) - https://netropolitan.co.nz/shop/chr-hansen-bactoferm-meat-starter-cultures/

Additional elements, as needed:
Magnesium - https://www.mrvitamins.com.au/products/nts-magsorb-mg-oil
Silica - https://www.swansonaustralia.com/solaray-bamboo-stem-extract-300-mg-60-vegcaps
(Bulk) https://www.amazon.com.au/BulkSupplements-Bamboo-Extract-Powder-Grams/dp/B01LVZW6Z7
Iodine - https://www.ariyahealth.com.au/product/28698-ariya-purity-lugol-iodine-25ml
Chromium - https://www.gosupps.com/superior-source-chromium-nutritional-supplements-500-mcg-60-count.html
Disodium Phosphate - https://www.ebay.com.au/itm/152197693111

Foods, salts:
Sodium Chloride - https://www.woolworths.com.au/shop/productdetails/320992/mckenzie-s-natural-salt-sea
Potassium Chloride - https://ozdingo.com.au/products/pure-potassium-chloride-powder
Oat Bran - https://www.woolworths.com.au/shop/productdetails/203867/woolworths-oat-bran
Ginger - https://www.woolworths.com.au/shop/productdetails/680510/woolworths-crushed-ginger

STEP 3 - CALCULATE SUPPLEMENTS / DOSES
It is generally expected that ACTUAL deficiencies for silicon, magnesium, iodine, selenium, molybdenum and others may show in your Oligoscan data and that FUNCTIONAL deficiencies for iron, manganese, copper and six others may exist in the HTMA, indicating inflammation severity over time. These may take some months to restore. Recheck your Oligoscan for progress on remineralisation monthly (and HTMA, as desired, for progress on inflammation.) Adjust supplements as needed. Where a mineral is non-optimal, you can use these suggested (elemental) total daily doses below, based on the column the bar reaches.

(“So-Check” branded reports have a different formatting and show a “percentage deviation” from “normal”. You can ignore that chart’s two outer columns to use the dosing guide image above.)

Recheck every 4 weeks. If your Oligoscan profile shows extremely elevated levels of heavy metals (eg. lead, mercury or uranium), taking a final binder (eg. 5g of activated charcoal, micronized zeolite or bentonite clay) could be appropriate before bed and far away from meals. The included silica, spirulina, NAC, R-ALA and psyllium husk already have functions for this.

Elevations of electrolyte excretion in hair can indicate LOW cellular uptake / silicon deficiency. Where a HTMA profile shows low potassium and similarly low rubidium, the rubidium deficiency will need correcting or else potassium levels may be difficult to restore. Similarly calcium with strontium and magnesium with lithium. High zinc can indicate low histidine / insufficient protein.

NB. Consuming higher than recommended daily intakes of minerals where a deficiency is not present could create additional health problems and should be generally avoided. Consuming certain minerals on an empty stomach will easily induce nausea.

DIET AND ELECTROLYTES
Foods to generally consume as part of this protocol, per HTMA results:
Oat bran 40 g+ /day (silicon @ 143 mg/100g, beta glucans - increases sIgA, NK cells, inhibits glyoxylate shunt / isocitrate lyase),
Brazil and other nuts (silica, rubidium, strontium, cobalt, warning - high selenium),
Liver, organ meats (very nutrient dense, not every day), Bamboo shoots (silica),
Broccoli + radish / mustard seed (additional sulforaphane / NRF2, trace elements),
Cabbage (zirconium, rubidium), Carrots (Vitamin A), Green beans (silica),
Eggs (Iron, iodine, choline, lecithin, biotin, etc), Pumpkin seeds/oil (phosphorus),
Beetroot (nitrates for blood volume, nitric oxide and dopamine synthesis),
Ginger 2.5-5g/day (gut microbiome dysbiosis and itaconate / isocitrate lyase inhibition),
Pineapple (100-200 g for silicon, bromelain - fibrin clots). Generally avoid fructose.
High protein diet (essential amino acids, often good phosphate sources) >1.5g/kg/day
Low-GI carbs 1.5-2g/kg/day, unless employing a ketogenic diet (glycogen storage is also impaired when cortisol is elevated.) Gluten may be poorly tolerated at this stage and damage celia, increasing severity of symptoms.
Water 2.5-3L of water / day (detoxification, prevents red blood cell rouleaux formation.)

Other electrolytes (potassium, sodium, calcium, magnesium, phosphate, bicarbonate):
Maintaining electrolytes can be challenging, due to expected renal dysfunction. Electrolytes are required for ion channels / transporters and signalling pathways. Where deficient, neurological symptoms, muscle spasms and pain/inflammation may occur. Nutrient absorption may be impaired. Electrolytes should be consumed slowly over the day and typically with carbohydrates / food. Consult your doctor if suffering from any pre-existing kidney disease.

Total daily (elemental) targets from all food / supplement sources -
Sodium (Na): 2-2.5 g, eg. 5 g (teaspoon) of table salt.
Potassium (K): >5 g, eg. 900g / 3 large potatoes, or eg.10g of potassium chloride (Nu-Salt).
Magnesium (Mg): >750 mg. 1.5-2 g, where deficient.
Calcium (Ca): >750 mg. 1.5-2 g, where deficient
Phosphate (P): 1 g for maintenance, higher where deficient. eg. Meat, dairy, pumpkin seeds / pumpkin seed oil, red lentils, sunflower seeds, potatoes. Supplements which combine phosphate with various electrolytes, eg. calcium phosphate may be available in some regions.

Elemental weights, by compound, for calculating servings of various electrolytes:
Sodium Chloride (Cl): 39% Na, 61 % Cl | eg. 6.4 g = 2.5 g sodium, 3.9 g chloride
Potassium Chloride: 52% K, 48% Cl | eg. 9.6 g = 5 g potassium, 4.6 g chloride
Calcium Phosphate: 39% Ca, 20% P | eg. 3.8 g = 1500 mg calcium, 770 mg phosphorus
Sodium Phosphate Dibasic: 40% Na, 31% P | eg. 6.25 g = 2.5 g sodium, 1.9 g phosphorus
Monosodium Phosphate: 19% Na, 26% P | eg. 6.6 g = 1.25 g sodium, 1.7 g phosphorus

STEP 4 - START THE PROTOCOL
It is fully expected that people exploring this protocol are highly sensitive to supplements.
To help reduce expected paradoxical symptoms and herxheimer effects, from any improved immune activity, a starting order has been provided. Deviations from this starting order may induce avoidable and unpleasant symptoms, however you can space out the stages further, as needed. This protocol is intended to stimulate immune activity and should produce a “J-curve” result, making you “feel quite sick” at the beginning of each stage.

BEFORE YOU START

1) First assess, remediate and maintain your lymphatic system as described earlier. Take note of any tissues which have been or continue to be sore / stiff / inflamed - these will be expected to flare during immune activity. These may also be adjacent to other infected tissues.

2) Due to fulvic acid’s potent and desired ability to “liberate” and recirculate metals that are being sequestered during chronic inflammation, it is suggested that you test your response to a single drop of the the Good State Fulvic Ionic Man (multi-mineral) in a glass of water before starting Stage 3 of the protocol. If you experience any unpleasant effects, take a few days to titrate up from a smaller dose (eg. place a drop in a bottle of water, mix and then transfer a drop from this first dilution into another glass of water) until you can consume a standard serving without observing these effects.

3) If you have nasal inflammation or notice a bottleneck when breathing in, performing a MARCoNS test through your health practitioner could be appropriate. Candida, Aspergillus, Staphylococcus and Klebsiella species are unwanted microorganisms sometimes found in the nasopharynx. These tests may also indicate susceptibility of detected strains to interventions like fluconazole, itraconazole, voriconazole, amoxicillin, etc.

4) If your intracellular magnesium, zinc, selenium, iodine, or calcium levels are low, you may experience additional “B6 toxicity” symptoms which would be enhanced by consuming additional B6 in any form. Gut motility issues are also commonly experienced.

You may help avoid unwanted symptoms by FIRST consuming the food items and electrolytes to reach the suggested total daily intakes for 7 - 10 days.. One dose of the oral magnesium complex and liberally applying the magnesium gel 2+ times / day covering armpits to elbows, stomach, etc. can be highly effective.

NB. Correcting severe electrolyte deficiencies can create temporary paradoxical effects and increased rates of metabolism, heart rate, etc.

5) Where described below, “x 3” means “3 times per day” and not “3 doses all at once”.

Weeks 1-2+ (STAGE 1 - Remineralisation, increasing collagen synthesis rates and performing a controlled initial “die-off”)

Perform regular lymphatic maintenance, basic posture rehab and “pacing” as described above.

This stage can be initially unpleasant due to the microbial ‘die-off’ effect - even though it is being minimised. Slow things down, as needed / use reduced doses and build up. 100mg/day aspirin can be helpful at this stage to help inflammation / hypoxia.

Start consuming / absorbing the foods and electrolytes discussed in Step 3 & 4, then introduce the antiseptic intervention (expect die-off, etc), plus:

Enzymes -
“Anti-fungal enzymes 1” - Candex (Digests carbohydrates, cell walls, biofilms. You can consume up to 6 of these per day - 1 capsule, 15 minutes after food to help digest carbohydrates x 3. 1 capsule x 3, away from meals, to digest microbial cell walls. Start slowly.)

B Vitamins -
B1 - Thiamine 500 mg or TTFD 50mg (Acetaldehyde metabolism. Start 1/10th dose & increase.)
B2 - Liposomal Riboflavin 100 mg, as 2 x 25mg (small) scoops. (This will reduce over time.)
B3 - Niacinamide (no-flush) 250-500mg x 2 (NAD+ biosynthesis, immunity.)
B9 - Folinic Acid (NOT Folic) 800-1200 mcg x 1 (Methylation cycle, BH4.)
B12 - Vimergy x ¼-½ serving (sublingual)

Other -
PQQ 20mg, CoQ10 200 mg x 1-3 (DBH promoter, Electron Transport Chain support)
Vitamin C - 1000mg x 1 (HIF-1a, DBH, collagen synthesis. (Can take 1/2 , twice per day)
Acetyl L-Carnitine 1000 mg x 2 (Fatty acid transport, dopamine synthesis promoter)
Vitamin D3 5000 IU - 8000 IU x 1 (PNMT modulation, immunity, GLUT2.)
DHA 300-500 mg x 1 (Lipid membrane maintenance.)
R-ALA 100 mg x 1 (AMPK promoter.)
Apigenin eg. 50mg x 1 AM (ALDH, p38 MAPK inhibitor, TGF-b1 modulator and NADase inhibitor. Start at ¼ dose and increase. Upper threshold for effective dose may be 400mg/day.)

Amino Acids (you can pre-mix for easy dosing. Amino acid transporters saturate at around 15g/hour in some studies - best away from food.) -
Taurine 1-3g x 3 (GLUT2, glycogen synthesis)
L-Glutamine 2.5 - 5g (½ - 1 teaspoon) x 2 (Collagen synthesis rate, promotes IFN-gamma)
L-Lysine 1g x 2 (Collagen synthesis rate, antiviral functions)
L-Serine 1g x 2 (Neuropathy, neurotransmission)
D-Ribose 2.5g x 2 (½ teaspoon) (NAD+ biosynthesis, mitochondrial metabolism)
Creatine 2.5g x 2 (½ teaspoon) (GLUT4, ATP, SAMe metabolism efficiency)
Minerals -
Silica 1-2 capsules x 3, or more (Plus silica rich foods. HIF-1a. Antiviral. Magnesium uptake. Collagen. Silica will also be heavily used to excrete acetaldehyde and toxic metals.)
Iodine approx 500-2000 mcg in water (as needed) (Thyroid, riboflavin metabolism.)

Probiotics -
Youtheory Spore Probiotic5 2 capsules (Microbiome, GI tract repair, endotoxemia.)
L. reuteri Probiotic5 x 1 (Helps prevent unwanted species dominating.)
LGG + Bifido Probiotic x 1-2 (Microbiome, GI tract repair, endotoxemia.)
L. sakei - apply to affected mucosal tissues, after completing the antiseptic phase.

5If considering the “Salt/Iodine GI Overgrowth Flush” in the beginning of the protocol, these colonising probiotics would be wasted and would be better included after any GI flush phase.

Morning -
(Before any food)
Psyllium Husk “shake” - a teaspoon of powder in a glass of water. (Will assist toxin removal, gut microbiome / GI motility.)
Spirulina6 - 500mg (1/5th tsp) morning
(After any food)
Zinc (if indicated by Oligoscan) +
EGCG (Reduces nitrogen metabolism, inhibits 11HSDβ1, increases zinc absorption)

Lunchtime, after food -
Copper (if indicated by Oligoscan)
Curcumin6 150mg / ½ capsule (Start slowly. Will inhibit microorganisms and promote MC1. Inhibits 11HSDβ1. Expect herx. Lower doses can promote NRF2 / decrease inflammation. Higher doses can increase IFN-gamma / symptoms by decreasing cortisol release.)

Evening, after food -
Zinc (if indicated by Oligoscan) +
EGCG (Reduces nitrogen metabolism, increases zinc absorption)
ANY other major deficiencies indicated by Oligoscan (or via HTMA data - rubidium, strontium, where indicated.)
Curcumin6 150mg / ½ capsule (Start slowly. Will inhibit microorganisms and promote MC1. Inhibits 11HSDβ1. Expect herx. Lower doses can promote NRF2 / decrease inflammation. Higher doses can increase IFN-gamma / symptoms by decreasing cortisol release.)

6NB. Drugs metabolised by CYP2D6 may have altered pharmacokinetics - making them more or less potent. Your doctor can best advise on how to manage any tapering or other dose adjustments.

Weeks 3-4+ (STAGE 2 - Increased GI / Mucosal Intervention)
Continue items in all previous weeks and;

Add -
A-GPC Choline >250 mg x 2
For GI motility and muscle contraction.
If muscle stiffness or headache occurs, reduce or pause dosing.

Aloe Vera juice 50ml x2 (Start slowly.)
Will increase sIgA, however in higher doses it can increase primary sex hormone production and therefore decrease cortisol / increase IFN-gamma activity, inflammation, which isn’t desirable until the minerals are optimal. Pause / reduce as needed. Expect die-off / herxheimer symptoms - headache, rash, sore joints, etc.)
Biotin 300-600 mcg x 1, for 1 week ONLY. (Only take if indicated by OAT results.)
Required for normal energy metabolism, however will feed fungal infections and promote fungal bloom, if infections are not sufficiently inhibited / managed.

Then -
Slowly increase the morning spirulina dose to 1g (1/5th tsp) - 5g (1 tsp) of Spirulina7.
Slowly increase the evening curcumin dose to 300 mg / 1 capsule7, if tolerable. If IFN-gamma increases too much and fatigue / tachycardia / adrenaline increases significantly, reduce to a tolerable dose and pause there until silicon, magnesium, copper and manganese are optimal.

(7These both increase immunomodulation for the IFN-gamma pathway and inhibit pathogens, histamine. May initially cause nausea, headache, fatigue from fungal die-off symptoms - take this slowly and adjust as tolerable. )

Once these supplements settle in, it would be a good time to start testing / increasing your tolerance to the fulvic acid in the Good State Ionic Man, starting at 1 drop in a glass of water per day and slowly increasing up to 1 serving / day.

During inflammation, adding fulvic acid will “liberate” sequestered metals and recirculate them. This has been described as “rapidly removing a band-aid from every cell at the same time”. Increase from 1 drop very slowly over 1-2 weeks.

Weeks 5-8+ (STAGE 3 - Stimulate and support strong immune response)
[NB. Confirm successful remineralisation via Oligoscan before proceeding to this stage.]

Continue items in all previous weeks APART FROM minerals now measuring as “Normal+” on the Oligoscan report and then add (and expect die-off symptoms, heavy immune response, pain / inflammation, POTS, fatigue, etc):

If your cortisol / heart rate becomes elevated for long periods (eg. >6 hours), or immune-related tissue pain/inflammation extends beyond 3 days per localised tissue, take a few days off the mushrooms, double the Vitamin C and simultaneously decrease spirulina to 500mg + curcumin to 1/2 doses during this “recovery break”.

NAC (Thrombolytic, biofilm breaker and metal chelator - weeks 5,6,7 at 1000 mg x 3, then reduce to < 500 mg x 1) (Will cause die-off)
ACV 2-3 caps x 3 (may cause temporary GI upset, histamine increase.)
Life Extension Reishi Complex8 x 2 (Immunomodulation, targets various pathogens)
(8A 1-3 day initial headache is possible when starting reishi.)
Beta-glucans >250mg x1 AM (Increase NK cells. May increase histamine / insomnia, low dose.)

Morning -
(Before Food)
“Biofilm Breaker” - Biofilm Phase-2 Advanced x 2 capsules, 4 days/week. Start at ¼ capsule. This product can cause intense die-off. Please read product instructions. (Contains black seed oil, ALA, bismuth subnitrate.)
(After Food)
Good State Fulvic Ionic Man Multi Mineral x 2 servings (Start with low dose.)

Lunchtime, after food -
ALTERNATE EACH DAY, AS INDICATED BY YOUR OLIGOSCAN, EITHER
Copper OR
Good State Fulvic Ionic Iron x 2-4 servings (Start with low dose.)

Evening, after food -
Good State Fulvic Ionic Man Multi Mineral x 2 servings (Start with low dose.)

STEP 5 - OPTIONAL FOLLOW UP
When the element levels reported by your Oligoscan are optimal, a 3 day “water fast” may provide additional benefits. This is NOT advisable with severe mineral deficiencies.

If this is your first water fast, it is generally advisable to have someone monitoring you during this time. Avoid driving vehicles, stress and excessive exertion.

Fasting protocol requirements (per day):
No food or other supplements.
3L water.
5g (teaspoon) of sodium chloride (table salt = 2.5g sodium elemental)
3g NAC.
1000mg Magnesium oxide (will induce diarrhoea)

(Optional add-ons)
500mg EGCG x 39
Resveratrol 120mg x 3
Hesperidin 500mg x 2
“Salt/iodine Overgrowth Flush recipe” in Gastrointestinal Biofilms, Pathogens and Remediation

(9NB. Important safety consideration: To break a water fast “early”, use 2 or more Apple Cider Vinegar doses - without capsules - as a succinate source.
This is only a requirement if EGCG has been dosed in the previous 6 hours.

Failure to observe this safety consideration may result in rapid cyclical blackouts and convulsions until the EGCG is fully metabolised or until someone else administers Apple Cider Vinegar.
This could easily be fatal if eg. operating a motor vehicle. YOU HAVE BEEN WARNED.)

[v3.57 changelog]

+[Text / description updates], cleaned up product links in antiseptic vs main supplements section, probiotics, chlorella, gluten, others
+Updated antiseptic recipe
+Updated links for silica AU, USA, mag AU, BP2A AU, iodine USA
+Re-introduced phosphate links AU,USA,EU
+L. Sakei
+Biotin + warning