Research Group for ME/CFS, Chronic Disease, Aging and Cancer

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v3.51U (preview)
« on: April 09, 2022, 08:43:52 PM »
General Mitochondrial and Wellness Protocol
(Author: Joshua Leisk, V3.51U - 31st December 2022)

This is a dietary supplement based protocol which may be helpful for supporting the unique nutritional requirements of people with:

Myalgic Encephalomyelitis / Chronic Fatigue Syndrome (ME/CFS).
Long COVID / Long Haulers, Viral / Post-Viral Chronic Fatigue.
Breathlessness, Hypoxia, Acidosis, Alkalosis / pH Dysregulation.
Dysregulated Cortisol, Dyslipidemia, Alopecia.
Small Fibre Peripheral Neuropathy (SFPN).
Mast Cell Activation Syndrome (MCAS).
Diamine Oxidase (DAO) Deficiency and Elevated Histamine.
Brain Fog. Sleep disorders.
Major and other types of Depression. Anxiety.
Postural Orthostatic Tachycardia Syndrome (POTS).
Chronic Inflammatory Response Syndrome (CIRS), Fibromyalgia.
Frequent Urination.
GI Disorders, Small Intestinal Bowel Overgrowth (SIBO).
B6 Toxicity.
Ehlers Danlos Syndrome (EDS), Joint Pain, Connective Tissue Disorders, Tinnitus.
Polycystic Ovary Syndrome (PCOS).
Erectile Dysfunction.
Elevated Cholesterols / Fasting Glucose / D-Dimer.

NB. While all efforts are continuing to be made in eliminating them, some temporary paradoxical effects may be observed during protocol commencement. As certain deficiencies are corrected and neurotransmitter homeostasis is reset, paradoxical effects may include: temporary adrenaline and heart rate increases..

Existing "burning" sensations and headaches may also “flare”. Temporary alterations to libido may be expected. Resolving a severe copper deficiency may induce temporary kidney pain and nausea in some cases. Improvements to adaptive immune function are expected to occur during this protocol, which may result in some short periods of productive, yet otherwise unpleasant tissue inflammation and/or fever, flu-like symptoms, fatigue, etc.

Monitoring Lactate Dehydrogenase (LDH) - target 100-150 mmol/L, D-dimer, cholesterols and fasting glucose via blood tests would be suggested.

Disclaimer: Always consult with your doctor, especially if taking medications, eg. SNRI /SSRI, beta blockers, MAO inhibitors, etc.

The human body contains a large number of elements. Many of these elements have been well-documented as having important biological activity in humans.

Our ongoing research has identified a persistent / cyclic inflammatory cascade, relating to immune dysfunction, which directly causes insufficiency of at least 9 important elements that use a common pair of transporters - Divalent Metal Transporter 1 and Ferroportin. Further deficiencies are created in the metabolic cascade being described.

However, some variability exists in the pattern of insufficiencies. This can be due to alterations in renal function and general homeostasis by complex relationships between the 9 affected elements and some seemingly unrelated heavy metals, along with additional trace elements. These complex relationships require further research.

The metabolic cascade appears in a surprising number of infections and chronic diseases. The model describes severe mitochondrial dysfunction, hypoxia, neurological alterations, collagen synthesis issues and immune deficiency. This is not an exhaustive list. The pattern of elemental deficiencies are also predictive of various disease features, when mapped to impairment of  related enzymatic reactions. A preview of the disease model and biochemistry pathways being described in our upcoming paper have been made available on the website and Discord server.

Correcting these nutritional deficiencies via diet and/or supplements can be extremely difficult due to the cyclic nature of the disease model and impaired absorption. Ongoing improvements to solving these deficiencies are reflected in protocol updates.

Complementary Therapies
(A short note on the general importance of maintaining a functioning lymphatic system.)

The lymphatic system is a network which spans the majority of the human body. Quite surprisingly, until very recently we were not aware that this also included the brain -
The lymphatic system has a number of important roles in immunity and circulation.

Lymphoid organs are the source of B and T lymphocytes. The system also collects extracellular fluid and metabolites from tissues, returning them via lymphatic vessels to the bloodstream, preventing lymphoedema or catastrophic fluid buildup and metabolic backlog.

A friendly primer on the lymphatic system can be found here -

The lymphatic system relies on breathing and muscle contractions to maintain healthy circulation and waste excretion.

In any disease where movement is being restricted, this creates additional difficulties for maintaining immune functions and circulation.

Manual therapy may compensate for this.
A regular session with a massage therapist who specialises in lymphatic drainage may help you assess and remediate any recurring lymphatic blockages. These may also be observed as being stiff, sore swollen tissues that can feature enlarged lymph nodes, such as in the neck, groin or armpits. A strong paradoxical effect may be felt the first time a blockage is cleared.

A daily regimen to maintain any problematic areas by hand or vibrating massage gun (used on a glancing angle), may also assist unlocking functional gains and preventing pain in chronic illnesses / infections. Conversely, not maintaining the lymphatic system may create a roadblock.


A baseline Hair Toxin Mineral Analysis (HTMA) is required to obtain an approximation of your mineral status and personalise mineral supplementation. For this purpose, a list of vendors offering a compatible list of markers and reference ranges is provided here:

Doctors Data and other compatible laboratories:
AU - (can manually add rubidium)
EU - ($try UK site first)
EU - (doesn't include rubidium)
EU - (doesn't include rubidium)
NZ -
UK, some EU -
US - (Not available in NY/ NYC and other areas.)

Online resellers for Trace Elements Inc (doesn’t include iodine, optional boron):
AU -
DE -
FR -
NZ -
PL -
SG -
UK -
US & CAN -

Hair collection guide:
1. Please ignore any conflicting vendor-specific instructions.
2. The hair needs to be clean, well-rinsed, dry, untreated and uncoloured. Unwashed hair may provide false (elevated) data for sodium and potassium, from dried sweat.
3. Select suitable areas of sideburn, scalp and/or neck hair. If the hair is longer than 2.5cm / 1", first trim the sample area to this maximum length with clean stainless steel scissors. By using shorter hair, the report data relates to a more recent period of time.
4. Cut the (remaining) hair to be sampled as close to the skin as possible. Do not use clippers or an electric razor - metal contamination from tungsten blades, etc may occur. Collect at least 2 heaped tablespoons worth of hair, or approximately 250 mg. If the amount of hair provided is insufficient, the sample may be rejected.
5. Place the hair sample in the sample kit envelope provided, or in a clean, clearly labelled envelope or sachet. Whilst Trace Elements Inc says not to use ziplock bags as apparently hair “sweats” and the sample may be rejected, Doctors Data supplies ziplock bags in their kit.
6. Repeat the HTMA every eg. 6 weeks, using fresh regrowth from the same area, until an optimal mineral balance is achieved.


It will typically take 2-3 weeks to get your HTMA results and a potentially similar timeframe to order / receive supplements from any overseas vendors. Now would be an opportunistic time to order any/all supplements which do not require personalisation by HTMA results and presumptively, at least the 3 mineral supplements which are used early in the schedule. Expect 3+ months to remineralise from severe mineral deficiencies, although this is improving.

Some products are not available domestically in all regions and will need to be imported from the US. As such, the “master list” is based around US suppliers. However, a list of local vendors / supplements for regions has been provided for some products. It is expected that local lists will expand over time. (PRODUCTS RECENTLY UPDATED are marked in GREEN)

[USA / Master List 1/2 - most products ship internationally]
Standard supplements, regardless of HTMA:
Liposomal Riboflavin* -
(*This is a unique product - do not substitute. If you buy 2, a cheaper shipping option appears and the total order price doesn’t change. Digestion and absorption / transport bottlenecks exist for riboflavin. Multiple alternate forms have been tested without success. This microencapsulated product bypasses these issues.)
Liposomal or sublingual NAD+ precursor (standard oral NAD+ precursors don’t absorb well) -
Vitamin C + D-Ribose -
[iHerb Quicklist for this section - ]
Berberine -
PQQ + CoQ10 -
Thorne Basic B Complex* -
(*Do not substitute. This B complex product has been carefully selected to contain sufficient amounts of the required forms of each coenzyme, minus inappropriate amounts of pyridoxine, yet adequate P5P.)
Folinic Acid (not folic acid) -
Vitamin D3 -
Vitamin A -
A-GPC Choline -
Forskolin -
Artichoke / Luteolin -
Apigenin -
Reishi* -
(*PRODUCT DOES NOT SHIP TO EU - use EU source. Do not substitute. A USP study found approximately 75% of reishi products are fake. This product passed our HPLC + LCMS mass spectrometer testing. Most did not. Further, different metabolites are extracted from different parts of the fungi, eg. fruiting body, spores and mycelium. Beta-glucans require hot water extracts, triterpenes require alcohol extracts.)
Lions Mane + Ergothioneine* -
(*PRODUCT MAY NOT SHIP TO EU - if so, use EU source.)
Taurine -

“Standard” Elemental Supplements:
(For any Naturitas products, there is a mobile app available. You can also use or these links to redirect to your local region):

Fulvic acid “complexed” ionic multi-mineral (Boron, Chromium, Iodine, Lithium, Magnesium, Manganese, Molybdenum, Potassium, Rubidium, Selenium, Strontium, Zinc, others) -
Silicon (not tested by these HTMA reports, however a key elemental deficiency frequently observed) -
Calcium Phosphate (Marine) -
Magnesium (oral) -
* AND * (transdermal) -
(Transdermal absorption route appears to be significantly more effective in some people. This magnesium product has eucalyptus and menthol, providing additional benefits for energy metabolism and immune support.)

Additional elements, as indicated by HTMA:

Fulvic acid “complexed” Copper -
Fulvic acid “complexed” Iron -
Sulphur -
Vanadium -
Cobalt -
Bismuth -
Germanium GE-132 -

Foods, salts (use local sources):

Sodium Chloride  -
Potassium Chloride -
Ginger (ground) -
Oat Bran -

[EU / Europe - alternate / domestic sources for some products]
Standard supplements, regardless of HTMA:

Reishi -
Lions Mane -
PQQ + CoQ10 -

Additional elements, as needed:

Calcium + Phosphorus -
Potassium -

Foods, salts:

Sodium Chloride -
Potassium Chloride -
Oat Bran -
Ginger -

[AU / Australia - alternate / domestic sources for some products]
Standard supplements, regardless of HTMA:

Taurine -
PQQ + CoQ10 -

Additional elements, as needed:

Magnesium (transdermal) -

Foods, salts:

Sodium Chloride -
Potassium Chloride -
Oat Bran -

[Supplements currently in testing for future updates, known benefits]
Rosemary Extract - glucose uptake, catecholamines, ACh, muscle preservation, anti-inflammatory, increases catecholamines
Boswellia - anti-inflammatory
PEA - anti-inflammatory
Bitter Melon - anti-inflammatory, increased acyl-coa dehydrogenases, SOD promotion
Fulvic “Trace Elements” - potential replacement for the additional elemental supplements


Except for electrolytes, adjust / remove / replace mineral supplements using your HTMA report as an approximate guide. These reports are unable to show eg. tissue-specific deficiencies.

The general expectation is that deficiencies for iron, manganese (and copper, typically where lead, mercury or uranium are measured low) will exist, along with various others. These may take some months to restore. Special forms of some elements may be required for efficient remineralisation. Recheck your HTMA every 4-6 weeks and adjust as needed. At 6 weeks, averaged results will approximate 3 weeks progress, assuming linear progress.

If your HTMA profile shows elevated levels of heavy metals (eg. lead, mercury or uranium), taking a final binder (eg. 5g of activated charcoal, micronised zeolite or bentonite clay) or 5g of Pectasol-C could be appropriate before bed and far away from meals. Join us online for help.

Where a HTMA profile shows low potassium and similarly low rubidium, the rubidium deficiency will need correcting or else potassium levels may be difficult to restore. Similarly calcium with strontium and magnesium with lithium. High zinc can indicate low histidine / insufficient protein.

NB. Consuming higher than recommended daily intakes of minerals where a deficiency is not present could create additional health problems and should be generally avoided. Consuming certain minerals on an empty stomach will quite likely induce nausea.

Foods to generally consume as part of this protocol, per HTMA results:
Brazil and other nuts (silicon, rubidium, strontium, cobalt, warning - high selenium)
Liver, organ meats (very nutrient dense, not every day)
Broccoli + radish / mustard seed (additional sulforaphane, trace elements)
Cabbage (zirconium, rubidium), Carrots (Vitamin A), Green beans (silicon),
Eggs (Iron, iodine, choline, lecithin, biotin, etc),
Beetroot (nitrates for blood volume, nitric oxide and dopamine synthesis),
Ginger 2.5-5g/day (gut microbiome dysbiosis and itaconate / isocitrate lyase inhibition),
Oat bran (40 g+ /day for beta glucans, silicon - immunity, itaconate pathway),
Pineapple (100-200 g for silicon, bromelain - fibrin clots). Generally avoid fructose.
High protein diet (essential amino acids, often good phosphate sources) >1.5g/kg/day
Low-GI carbs 1.5-2g/kg/day, unless employing a ketogenic diet.

2.5-3L of water / day to help prevent dehydration and red blood cell rouleaux formation. 

Other electrolytes (potassium, sodium, calcium, magnesium, phosphate, bicarbonate):
Maintaining electrolytes can be challenging, due to expected renal dysfunction. Electrolytes are required for ion channels / transporters and signalling pathways. Where deficient, neurological symptoms, muscle spasms and pain/inflammation may occur. Nutrient absorption may be impaired. Electrolytes should be consumed slowly over the day and typically with carbohydrates / food. Consult your doctor, if suffering from any pre-existing kidney disease.
Total daily (elemental) targets -
Sodium: 2-2.5 g, eg. 5 g (teaspoon) of table salt.
Potassium: >5 g, eg. 900g / 3 large potatoes, or eg.10g of potassium chloride (Nu-Salt).
Magnesium: >750 mg. 1.5-2 g, where deficient.
Calcium: >750 mg. 1.5-2 g, where deficient
Phosphate: 1 g for maintenance, higher where deficient. eg. Meat, dairy, red lentils, sunflower seeds, potatoes. Supplements which combine phosphate with various electrolytes, eg. calcium phosphate may be available in some regions.

Elemental weights, by compound, for calculating servings of various electrolytes:
Sodium Phosphate Dibasic | 32% sodium, 22% phosphorus
eg. 7.8 g = 2.5 g sodium, 1.7 g phosphorus
Trisodium Phosphate | 42% sodium, 19% phosphorus
eg. 6 g = 2.5 g sodium, 1.1 g phosphorus
Sodium Chloride | 39% sodium, 61 % chloride
eg. 6.4 g = 2.5 g sodium, 3.9 g chloride
Sodium Bicarbonate (Baking Soda) | 27% sodium (best not consumed near meals)
eg. 9.3 g = 2.5 g sodium + bicarbonate overdose (diarrhoea, in a single dose)
Potassium Phosphate | 55% potassium, 15% phosphorus
eg. 9 g = 5 g potassium, 1.4 g phosphorus
Potassium Chloride | 52% potassium, 48% chloride
eg. 9.6 g = 5 g potassium, 4.6 g chloride
Magnesium Monophosphate | 28% magnesium, 23% phosphorus
eg. 2.7 g = 750 mg magnesium, 600 mg phosphorus
Calcium Phosphate | 39% calcium, 20% phosphorus
eg. 3.8 g = 1500 mg calcium, 770 mg phosphorus


It is fully expected that people exploring this protocol are highly sensitive to supplements.
To help avoid paradoxical symptoms that aren’t relating to improved immune activity, a starting order has been provided. Deviations from this starting order may induce avoidable and unpleasant symptoms, however you can space out the stages further, if needed.


1) First assess, remediate and maintain your lymphatic system. Take note of any tissues which have been or continue to be sore / stiff / inflamed - these will be expected to flare during immune activity.

2) Due to fulvic acid’s potent and desired ability to “liberate” and recirculate metals that are being sequestered during chronic inflammation, it is suggested that you test your response to a single drop of the the Good State Fulvic Ionic Man (multi-mineral) in a glass of water before starting the protocol. If you experience any unpleasant effects, take a few days to titrate up from a smaller dose (eg. place a drop in a bottle of water, mix and then transfer a drop from this first dilution into another glass of water) until you can consume a standard serving without observing these effects.

3) If you test a nasal application of the NAD+ spray and experience a temporary burning sensation in your nose / throat / ears, you may have a localised infection in those tissues. Performing a MARCoNS test through your health practitioner could be appropriate. Candida, Aspergillus, Staphylococcus and Klebsiella species are unwanted microorganisms sometimes found in these tissues. NAD+, Berberine and NAC may provide additional benefits against these pathogens, by inhibiting their energy availability, breaking down biofilms and other mechanisms..

NB. An unpleasant “herxheimer-like” effect would be expected during any increased immune response activity.

4) If your intracellular magnesium levels are low, you may experience “B6 toxicity” symptoms which would be enhanced by consuming additional B6 in any form. Gut motility issues are also commonly experienced.

You may help avoid unwanted symptoms by first consuming electrolytes to reach the suggested total daily intakes for 7 - 10 days, along with the Good State Ionic Man multi-mineral, before commencing the standard protocol. Liberally applying the magnesium gel 2+ times / day covering armpits to elbows, stomach, etc. can be highly effective.

NB. Correcting severe electrolyte deficiencies can create temporary paradoxical effects and increased rates of metabolism.

5) Where described below, “x 3” means “3 times per day” and not “3 doses all at once”.

Days 1-3+
Start (or continue) consuming / absorbing the foods and various electrolytes discussed in Step 3, plus:
Liposomal Riboflavin 200-250 mg, 2 x 2 large scoops, or 1 large scoop, x4. (This will reduce over time, as manganese / copper / selenium levels increase.
Liposomal NAD+ intranasal 2 sprays x 2-3
PQQ 20mg, CoQ10 200 mg x 1-3
Acetyl L-Carnitine 500-1000 mg x 2
Vitamin D3 5000 IU - 8000 IU x 1
Vitamin A 3000 mcg x 1 (or eat carrots)
DHA 300-500 mg x 1
Silica x 1 serving (or eat cooked green beans)
Berberine 500-1000mg x 3 (15-30 mins before / after meals)

Morning, after food -
Good State Fulvic Ionic Multi Mineral x 1 serving (Days 1,2,3+)
Taurine - 500mg - 3000mg (caution if using beta-blockers - reduces epinephrine release)
Lunchtime, after food  -
Good State Fulvic Ionic Multi Mineral x 1 serving (Days 2,3+)
Good State Fulvic Ionic Copper x eg. 2-4 servings as one dose
Taurine - 500mg - 3000mg (caution if using beta-blockers - reduces epinephrine release)
Evening, after food -
Good State Fulvic Ionic Multi Mineral x 1 serving (Days 3+)
Taurine - 500mg - 3000mg (caution if using beta-blockers - reduces epinephrine release)
+ Vanadium, Cobalt, Bismuth, Germanium, Sulphur (where indicated by your HTMA)

Days 4-6+
Continue taking the items in all previous days and add:
Thorne Basic B Complex 1 cap x 1-3 (Can expect to reduce this over time as key minerals are restored. Excess can cause peripheral neuropathy, via B6 / P5P.)
Folinic Acid (NOT Folic) 800-1200 mcg x 1
Vitamin C + D-Ribose x 1
EGCG 100-200 mg x 2-3
A-GPC Choline >250 mg x 2 (If muscle stiffness or headache occurs, reduce or pause dosing.)

Before bed, with food  -
Good State Fulvic Ionic Multi Mineral x 1 serving (total of 4+ doses/day)

Days 7+
Continue taking the items in all previous days and add:
R-ALA  (First 3 weeks at 100 mg x 3, then reduce to 100 mg x 1.)
NAC (Thrombolytic, biofilm breaker and mineral chelator - first 3 weeks at 1000 mg x 3, then reduce to < 500 mg x 1 until selenium is in range.)

Forskolin 10 mg x 1-2 (Temporary adrenergic increase, reduced histamine.)
Artichoke (Luteolin) 25 mg x 1 (Temporary adrenergic increase, reduced histamine.)

TTFD 50mg x 1 (Increase dose from 1/10th capsule, temporary neuro symptoms expected.)
ACV 2-3 caps x 3 (may cause temporary GI upset, histamine increase.)

Days 10+
Continue taking the items in all previous days and add:
Life Extension Reishi Complex* x 2
*A 1-3 day initial headache is possible when starting reishi.
Lions Mane (beta glucans) >250mg x1 AM (may increase histamine / insomnia, low dose.)
Apigenin eg. 50mg x 1 AM (p38 MAPK inhibitor and NADase inhibitor, start at ¼ dose and increase. Upper threshold for effective dose may be 400mg/day.)

Evening, with food (elemental amounts, only where indicated by your HTMA results) -
Good State Fulvic Ionic Iron x 2-4 servings

With "anaemia of inflammation", iron, manganese, copper and other mineral absorption is blocked in the GI tract and other cells at DMT1 and ferroportin. Alternate mineral forms / sources which do not use these transporters are needed. An IV infusion may be appropriate, where indicated by your healthcare professional. Any rapid ferritin increase can cause or accelerate hypophosphatemia. Care should be taken to consume optimal levels of phosphates, especially where already deficient. If an active eg. bacterial / fungal infection is present, these are best resolved before starting iron supplementation.


When the element levels reported by your HTMA are optimal, a 3 day “water fast” may provide additional benefits. This is NOT advisable with severe mineral deficiencies.

If this is your first water fast, it is generally advisable to have someone monitoring you during this time. Avoid driving vehicles, stress and excessive exertion.

Fasting protocol requirements (per day):
No food or other supplements.
3L water.
5g (teaspoon) of sodium chloride (table salt = 2.5g sodium elemental)
1g of baking soda (110 mg of sodium)

(Optional add-ons)
500mg EGCG x 3*
Resveratrol 120mg x 3
Hesperidin 500mg x 2

(*NB. Important safety consideration: To break a water fast “early”, use 2 or more Apple Cider Vinegar doses - without capsules - as a succinate source.
This is only a requirement if EGCG has been dosed in the previous 6 hours.

Failure to observe this safety consideration may result in rapid cyclical blackouts and convulsions until the EGCG is fully metabolised or until someone else administers Apple Cider Vinegar.
This could easily be fatal if eg. operating a motor vehicle. YOU HAVE BEEN WARNED.)

« Last Edit: January 02, 2023, 04:39:10 PM by joshua.leisk »
NB. I am NOT a doctor and all information provided is for educational purposes only.

Please consult your physician before attempting anything you read here.