Research Group for ME/CFS, Chronic Disease, Aging and Cancer

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v3.51U (preview)
« on: April 09, 2022, 08:43:52 PM »
General Mitochondrial and Wellness Protocol
(Author: Joshua Leisk, https://bornfree.life V3.52U - 4th February 2023)

This is an experimental protocol which may be helpful for supporting the unique nutritional and wellness / lifestyle requirements of people with:

Myalgic Encephalomyelitis / Chronic Fatigue Syndrome (ME/CFS).
Long COVID / Long Haulers, Viral / Post-Viral Chronic Fatigue.
Migraines. Breathlessness, Hypoxia, Acidosis, Alkalosis / pH Dysregulation.
Dysregulated Cortisol, Dyslipidemia, Alopecia.
Small Fibre Peripheral Neuropathy (SFPN).
Mast Cell Activation Syndrome (MCAS).
Diamine Oxidase (DAO) Deficiency and Elevated Histamine.
Brain Fog. Sleep disorders. Major and other types of Depression. Anxiety.
Postural Orthostatic Tachycardia Syndrome (POTS).
Chronic Inflammatory Response Syndrome (CIRS), Fibromyalgia.
Dysautonomia. Frequent Urination. GI Disorders. Small Intestinal Bowel Overgrowth (SIBO).
B6 Toxicity. Candidiasis. Aspergillosis. Ehlers Danlos Syndrome (EDS), Joint Pain, Connective Tissue Disorders, Tinnitus. Polycystic Ovary Syndrome (PCOS). Erectile Dysfunction.
Elevated Cholesterols / Fasting Glucose / D-Dimer.


NB. While all efforts are continuing to be made in eliminating them, some temporary paradoxical effects may be observed during protocol commencement. As certain deficiencies are corrected and neurotransmitter homeostasis is reset, paradoxical effects may include: temporary adrenaline and heart rate increases. Resolving a severe copper deficiency may induce temporary kidney pain and nausea in some cases.

Herxheimer effects from microbial die-off can also be experienced. Existing "burning" sensations and headaches may also “flare”. Temporary alterations to libido may be expected. Improvements to adaptive immune function are expected to occur during this protocol, which may result in some short periods of productive, yet otherwise unpleasant tissue inflammation and/or fever, flu-like symptoms, nausea, fatigue, etc.

Monitoring Lactate Dehydrogenase (LDH) - target 100-150 mmol/L, D-dimer, cholesterols and fasting glucose via blood tests would be suggested.

Disclaimer: This protocol contains educational / general information ONLY. Always consult with your doctor to make sure this information is appropriate for you, especially if taking medications, eg. SNRI /SSRI, beta blockers, MAO inhibitors, etc.

From v3.52 onwards, this protocol now includes a significant shift upstream in the cascade, targeting the pathogenic landscape and immune dysregulation.


INTRODUCTION

The human body contains a large number of elements. Many of these elements have been well-documented as having important biological activity in humans.



Our ongoing research has identified an IFN-α:IGN-γ immune dysfunction and persistent / cyclic inflammatory cascade that causes a functional insufficiency of at least 9 important elements that use a common pair of transporters - Divalent Metal Transporter 1 and Ferroportin. Further systemic deficiencies are observed in the metabolic cascade being described.

However, some variability exists in the pattern of insufficiencies. This can be due to alterations in renal function and general homeostasis by complex relationships between elements and some seemingly unrelated heavy metals. These complex relationships require further research.

The metabolic cascade appears in a surprising number of infections and chronic diseases. The model describes severe mitochondrial dysfunction, hypoxia, neurological alterations, collagen synthesis issues and immune deficiency. This is not an exhaustive list. The pattern of elemental deficiencies are also predictive of various disease features, when mapped to impairment of  related enzymatic reactions. A preview of the disease model and biochemistry pathways being described in our upcoming paper have been made available on the website and Discord server.

Separate to the functional insufficiencies from inflammation, other testing methodology has shown a pattern of systemic mineral deficiencies which impair immune functions. Measuring, let alone correcting these nutritional deficiencies via diet and/or supplements can be extremely difficult due to the cyclic nature of the disease model and impaired absorption. Ongoing improvements to solving these problems are reflected in protocol updates.


LYMPHATIC MAINTENANCE REGIMEN
(A short note on the general importance of maintaining a functioning lymphatic system.)
The lymphatic system is a network which spans the majority of the human body. Quite surprisingly, until very recently we were not aware that this also included the brain -
https://www.nih.gov/news-events/nih-research-matters/lymphatic-vessels-discovered-central-nervous-system
 
The lymphatic system has a number of important roles in immunity and circulation.

Lymphoid organs are the source of B and T lymphocytes. The system also collects extracellular fluid and metabolites from tissues, returning them via lymphatic vessels to the bloodstream, preventing lymphoedema or catastrophic fluid buildup and metabolic backlog.

A friendly primer on the lymphatic system can be found here - https://www.britannica.com/science/lymphatic-system.

The lymphatic system relies on breathing and muscle contractions to maintain healthy circulation and waste excretion.

In any disease where movement is being restricted, this creates additional difficulties for maintaining immune functions, fat transport and circulation.

Manual therapy may compensate for this.
A regular session with a massage therapist who specialises in lymphatic drainage may help you assess and remediate any recurring lymphatic blockages. These may also be observed as being stiff, sore swollen tissues that can feature enlarged lymph nodes, such as in the neck, groin or armpits. A strong paradoxical effect may be felt the first time a blockage is cleared.

A daily regimen to maintain any problematic areas by hand or vibrating massage gun (used on a glancing angle), may also assist unlocking functional gains and preventing pain in chronic illnesses / infections. Conversely, not maintaining the lymphatic system may create a roadblock.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5954877/


LIVING WITHOUT CHRONIC YEAST, MOULD & FUNGAL INFECTIONS

Yeasts, moulds and fungi are found everywhere in nature and have an important role. They decompose animals, plants and other organisms, recycling their elements and other nutrients, thereby continuing the circle of life.

Usually these decomposing animals are not alive, however when an organism’s immune response is incapable of maintaining a defence against the normal daily background level of fungal challenge, the living organism will become food for yeast, mould and/or fungi. Various lifestyle influences contribute positively and negatively to maintaining this daily defence.

Lack of exposure to sunlight, lack of sufficient activity to maintain a specific immune function and/or lack of a protective microbiome can also contribute to an organism being more susceptible to mould. It has been previously said “we don’t grow old, we mould!”

The barrier function of the skin and mucosal layers play an important part in resilience against pathogens. Unfortunately, the human body has a number of tissues that are opportunistic for yeast / mould / fungal infections to thrive with minimal interference from direct immune activity.




Do you have a history of cracked lips / ears / toes / feet / rectum, flaking skin, dandruff, hair loss, red / inflamed skin patches / rashes, nasal inflammation, sleep breathing disorder, gut pain / issues, white or green/black film on tongue, discharge?

Orifices and tissues such as the nasopharynx, throat, ears, GI tract, rectum, vagina, feet, toes are areas which are not regularly exposed to direct sunlight. This list might well include all tissues, where someone is bed-bound or house-bound. These tissues provide “safe spaces” for any fungal spores to seed, colonise and thrive, without appropriate immune surveillance. Subgrouping of ME/CFS patients may have a relationship with the locations of infected tissues. In the case of a GI tract infection, this may also affect dietary absorption efficiency. The nasopharynx may be another important tissue. A healthy microbiome appears to play a very important role in providing protection against invasive yeast, mould and fungal infections.

Apart from generating gaseous toxins, yeasts / mould / fungi can cause dramatic metabolic alterations. They have an ability to break through our skin / mucosal barrier function from a realtively “safe space” and cause recurring / chronic immune activation during the invasion. This immune activation inhibits specific mitochondrial metabolism and reconfigures a number of other metabolic pathways toward combating these pathogens. However, while ultimately beneficial, these alterations can be quite debilitating under certain conditions.

Additionally, whenever our immune system is successful in killing these microorganisms, their death causes the circulation of other toxins which contribute to a well-known “die-off effect” occurring.

This die-off effect is responsible for a number of highly debilitating symptoms, such as headaches, nausea, additional fatigue, dizziness, swollen glands, bloating / gas, constipation or diarrhoea, joint or muscle pain, tachycardia, chills, cold hands / feet, itchiness, rashes, sweating and/or fever.

This can be an ongoing / recurring cycle of extreme unpleasantness, if these infections and their original “safe spaces” are not fully resolved and the layer of protective microbiome restored.

We have been actively researching a combination of some existing strategies to help kill these microorganisms and minimise the die-off symptoms. Some additional challenges relate to the lifecycle stages and biofilms being created, which provide further protection against our immune activities.

In addition to the supplement schedule included in this protocol, a more rapid clearance of any (somewhat) accessible microorganisms may be achieved by direct or topical interventions for these infected tissues. Commercial preparations are available with various levels of efficacy.

Popular antifungal soaps, shampoos and creams containing zinc pyrithione can be effective at inhibiting fungal overgrowth. However, these products are now banned in Europe due to concerns around causing DNA damage.
However, a successful intervention for these tissues may include first administering a solution to break up any protective biofilms, leaving this solution to incubate for 20-30 minutes before (where possible / appropriate) wiping the area clean and then administering a second solution to selectively inhibit these foreign cells. These 2 solutions can also be applied simultaneously. The process may be repeated daily for 1-2 weeks, or as necessary.

Ongoing testing and literature review has suggested that a solution of NAC and water alone can reliably break up many biofilms and has other beneficial functions.

Further research has also suggested that a solution containing diluted tea tree oil, clove oil and coconut oil, when applied with or immediately following NAC yields a tolerable, yet significant inhibitory effect on bacteria, yeasts, moulds and fungi which may rival or exceed existing pharmacological interventions and is appropriate for a range of applications.

Commercial preparations made from tea tree oil and clove oil, are readily available as shampoos, vaginal douches, topical sprays, mouthwashes, creams and suppositories.


DIY Antiseptic Nasopharynx Clean and Flush, Dental Rinse, Topical Spray Recipe:

Part 1:
Mix 500mg of NAC powder (not from a capsule - it usually has fillers) and 5 ml of water.

Part 2:
Separately, mix 1 ml tea tree essential oil (20 drops) and
0.1mL clove essential oil (2 drops) and
4 ml of coconut / MCT oil. (You can add more coconut oil if you find this too strong.)

[Assuming use as a 1-step intervention, mix both parts together to form your final solution. Store in an empty glass bottle with a dropper for convenient dosing.]

To test this as a nasal rinse - while laying on your back, with your head tilted back and breathing through your mouth, drip about 6 drops of the solution into each nostril.

Let the solution run into the nasopharynx and incubate, while you “suffer” through some “minor burning sensations” for a minute or so.. then blow / purge your nose, leaving the residue behind. Repeating nose purging / clearing the nasopharynx whenever it feels appropriate.

To test this as a dental rinse - brush / floss as usual, then apply a few drops to your tongue and gums. NB. This is not the nicest tasting dental rinse. Brushing immediately afterwards may damage tooth enamel.

To test this as a topical antiseptic - simply clean the area and then spray / apply the solution.

(Links to the various ingredients can be found in the list of products in Step 2.)


THE IMPORTANCE OF “PACING” OR “JUST ENOUGH ACTIVITY”

Anyone who has experience with ME/CFS is likely to have gained a significant appreciation for the symptoms associated with “crashing” and Post Exertional Malaise (PEM).

A daily fear that too much activity will ‘crash’ you and leave you with fatigue / malaise, flu-like symptoms and often tinnitus. A further fear that this may persist for days / weeks / months.

A hypothesis surrounding the mechanisms involved in PEM is being described in the upcoming paper, however the WIP diagrams are available on the website and Discord server.

Unfortunately, due to the trauma surrounding PEM, some people choose to severely limit their activity. This unfortunately has an impact on the capabilities of their immune system to deal with chronic infection.

One of the primary tools the immune system can use against microbial challenges is creating reactive oxygen species (ROS) to oxidase these pathogens.

For optimal immune response, “pacing” should always attempt to test the appropriate upper threshold for activity, each day - unless that threshold has been exceeded already. Over sufficient time, the alternative leads to “rotting”, which is wholly undesirable.

The combination of supplements here should help improve the exertion threshold and buffer against “crashing”. However, you may have already noticed that fungal “die-off” symptoms share some common features with PEM and crashing. This will add some challenges for identifying your upper limits for exertion.

NB. The upper limit for exertion will be artificially reduced when interferon-gamma is elevated and Complex I is inhibited, during any intense immune response. Increasing temperature elevates interferon-gamma levels potentially 10x.

Proceed carefully, however the phrase “no pain, no gain” absolutely applies.


STEP 1 - COLLECT YOUR DATA

a) Research suggests that a Hair Toxin Mineral Analysis (HTMA) may provide an approximation of your FUNCTIONAL mineral status, averaged over the period of follicle growth, sampled downstream of the transporter alterations from inflammation - where circulating minerals can be sequestered inside various brain, liver and kidney cells and consequently low in other cells. A list of vendors offering a compatible list of markers and reference ranges is provided here:

Doctors Data and other compatible laboratories:
AU - https://www.toxno.com.au/articles/heavy-metals/hair-tissue-mineral-analysis-hma-or-htma-in-australia/ (can manually add rubidium)
EU - https://eneviahealth.com/en/tienda/mineralograma-de-pelo-doctors-data/ ($try UK site first)
EU - https://www.biocoherence.eu/en/shop/htma/ (doesn't include rubidium)
EU - https://www.lifelinediag.eu/en/product/eha-standard-en/#products (doesn't include rubidium)
NZ - https://www.houseofhealth.co.nz/product/hair-toxic-mineral-analysis/
PL - https://analizawlosa.com.pl/produkt/analiza-wlosa/
UK, some EU - https://www.lauristonlabs.co.uk/product/hair-elements-doctors-data/
US - https://www.walkinlab.com/products/view/dd-hair-elements-profile-doctors-data (Not available in NY/ NYC and other areas.)

Hair collection guide:
1. Please ignore any conflicting vendor-specific instructions.
2. The hair needs to be clean, well-rinsed, dry, untreated and uncoloured. Unwashed hair may provide false (elevated) data for sodium and potassium, from dried sweat. Use gloves.
3. Select suitable areas of sideburn, scalp and/or neck hair. If the hair is longer than 2.5cm / 1", first trim the sample area to this maximum length with clean stainless steel scissors. By using shorter hair, the report data relates to a more recent period of time.
4. Cut the (remaining) hair to be sampled as close to the skin as possible. Do not use clippers or an electric razor - metal contamination from tungsten blades, etc may occur. Collect at least 2 heaped tablespoons worth of hair, or approximately 250 mg. If the amount of hair provided is insufficient, the sample may be rejected.
5. Place the hair sample in the sample kit envelope provided, or in a clean, clearly labelled envelope or sachet. Whilst Trace Elements Inc says not to use ziplock bags as apparently hair “sweats” and the sample may be rejected, Doctors Data supplies ziplock bags in their kit.

The results obtained from the HTMA data may be used to infer averaged intracellular mineral availability, downstream of any chronic inflammation, for the period of time in which the hair follicles grew. This is especially relevant for the 9 elements discussed in the introduction.

b) Research suggests that an Oligoscan report can provide an approximation of your ACTUAL mineral status, as inferred by the mineral content of the skin in your hand. This is an in-clinic test, so a local practitioner will be required. Your blood type apparently affects the calibration or ranges in the report, so this information will be requested when you visit a clinic.

Unfortunately, a comprehensive list of providers is not yet available, however efforts are being made to find and compile a list of practitioners in all regions. If you have a good (or bad) experience with an Oligoscan practitioner, please share these details with our community Discord server.

The set of elemental markers collected is almost the same as the Doctors Data HTMA, however it also includes silicon, fluorine and unfortunately does not include rubidium or strontium markers. These are not known to be directly affected by inflammation, meaning the HTMA data can be referenced to help supplementation planning. However, the Oligoscan report provides the most actionable set of objective markers to target when choosing mineral supplements.

Your results may look something approximately like this -


While not happy with the level of published evidence currently supporting this emerging technology, our testing showed a clear pattern of deficiencies in ME/CFS, Long Covid and C19 vaccine injured people, with minor variability, which was not observed in controls.

c) A Great Plains Organic Acids Test (OAT) is a comprehensive report on urine metabolites.
It is available through local vendors or potentially, this website - https://gpldirect-international.com/collections/popular-test/products/organic-acids-test-oat 

In addition to allowing interpretation of metabolic impairment from the patterns observed in these markers, page 1 of the report shows various markers for microbial interference. Elevation of yeast and fungal metabolites in OAT results have been seen consistently in our collected data, with matching symptoms.

A further MycoTOX test may provide further clarity on species, if required.


STEP 2 - ORDER YOUR SUPPLEMENTS

While the Oligoscan provides instant results, it will typically take 2-3 weeks to get your HTMA results and a potentially similar timeframe to order / receive supplements from any overseas vendors.

Now would be an opportune time to order any/all supplements which do not require personalisation and each of the mineral supplements which are used early in the schedule.

Some products are not available domestically in all regions and will need to be imported from the US. As such, the “master list” is based around US suppliers. However, a list of local vendors / supplements for regions has been provided for some products. It is expected that local lists will expand over time. (PRODUCTS RECENTLY UPDATED are marked in GREEN)

[USA / Master List - most products ship internationally]
Standard supplements, regardless of HTMA:
Liposomal Riboflavin* - https://www.solacenutrition.com/product/cyto-b2/
(*This is a unique product - do not substitute. If you buy 2, a cheaper shipping option appears and the total order price doesn’t change. Digestion and absorption / transport bottlenecks exist for riboflavin. Multiple alternate forms have been tested without success. This microencapsulated product bypasses these issues.)
Antifungal Enzymes - https://www.allstarhealth.com/f/pure_essence_labs-candex.htm
L-Serine - https://www.bulksupplements.com/products/l-serine?variant=32133424218223
[iHerb Quicklist for this section -  https://iherb.co/Z96YEtb ]
No-flush Niacin - https://iherb.com/pr/now-foods-niacinamide-500-mg-100-veg-capsules/704
Spirulina (Powder) - https://iherb.com/pr/kos-organic-spirulina-powder-13-5-oz-381-5-g/104647
Ribose - https://iherb.com/pr/lake-avenue-nutrition-d-ribose-powder-unflavored-10-6-oz-300-g/97386
Psyllium Husk (or powder) - https://iherb.com/pr/frontier-co-op-organic-psyllium-husk-powder-16-oz-453-g/31080
NAC - https://iherb.com/pr/now-foods-nac-600-mg-250-veg-capsules/694
ALCAR - https://iherb.com/pr/now-foods-acetyl-l-carnitine-500-mg-200-veg-capsules/15271
PQQ + CoQ10 - https://iherb.com/pr/natural-factors-pqq-10-pqq-20-mg-coq10-200-mg-30-softgels/105659
Sublingual B12 - https://vimergy.com/products/organic-b12
Thorne Basic B Complex* - https://iherb.com/pr/thorne-research-basic-b-complex-60-capsules/18791
(*Do not substitute. This B complex product has been carefully selected to contain sufficient amounts of the required forms of each coenzyme, minus inappropriate amounts of pyridoxine, yet adequate P5P.)
Folinic Acid (not folic acid) - https://iherb.com/pr/source-naturals-megafolinic-800-mcg-120-tablets/7735
Vitamin D3 - https://iherb.com/pr/now-foods-vitamin-d-3-125-mcg-5-000-iu-240-softgels/22335
DHA - https://iherb.com/pr/source-naturals-vegan-omega-3s-non-fish-epa-dha-300-mg-60-vegan-softgels/46882
EGCG - https://iherb.com/pr/now-foods-egcg-green-tea-extract-400-mg-180-veg-capsules/11598
A-GPC Choline - https://iherb.com/pr/now-foods-alpha-gpc-300-mg-60-veg-capsules/12803
Forskolin - https://iherb.com/pr/life-extension-forskolin-10-mg-60-vegetarian-capsules/49696
Artichoke / Luteolin - https://iherb.com/pr/jarrow-formulas-artichoke-180-veggie-caps/177
R-ALA - https://iherb.com/pr/allmax-r-ala-60-capsules/67927
ACV - https://iherb.com/pr/swanson-apple-cider-vinegar-625-mg-180-capsules/109045
Apigenin - https://iherb.com/pr/codeage-liposomal-apigenin-non-gmo-vegan-90-capsules/114120
Reishi* - https://iherb.com/pr/life-extension-reishi-extract-mushroom-complex-60-vegetarian-capsules/46279
(*PRODUCT DOES NOT SHIP TO EU - use EU source. Do not substitute. A USP study found approximately 75% of reishi products are fake. This product passed our HPLC + LCMS mass spectrometer testing. Most did not. Further, different metabolites are extracted from different parts of the fungi, eg. fruiting body, spores and mycelium. Beta-glucans require hot water extracts, triterpenes require alcohol extracts.)
Lions Mane + Ergothioneine* - https://iherb.com/pr/real-mushrooms-ergo-l-ergothioneine-60-capsules/113975
(*PRODUCT MAY NOT SHIP TO EU - if so, use EU source)
Spore Probiotic - https://iherb.com/pr/youtheory-spore-probiotic-6-billion-cfu-60-vegetarian-capsules/78522

“Standard” Elemental Supplements:
(For any Naturitas products, there is a mobile app available. You can also use https://www.naturitas.com or these links to redirect to your local region):

Fulvic Acid “complexed” Ionic Multi-Mineral (Boron, Chromium, Iodine, Lithium, Magnesium, Manganese, Molybdenum, Potassium, Rubidium, Selenium, Strontium, Zinc, others) - https://goodstate.com/collections/man/products/ionic-man-mans-liquid-ionic-multi-mineral
Silica (not tested by these HTMA reports, however a key elemental deficiency frequently observed) -
https://www.naturitas.com/#/dffullscreen/query=FNature%20Organic%20Silica
Calcium Phosphate (Marine) - https://iherb.com/pr/veglife-red-marine-calcium-90-tablets/70153
Magnesium (Oral) - https://iherb.com/pr/now-foods-magnesium-citrate-180-softgels/22552
* AND * (Transdermal) - https://iherb.com/pr/life-flo-magnesium-oil-sport-spray-8-fl-oz-237-ml/49247
(Transdermal absorption route appears to be significantly more effective in people with SIBO. This magnesium product has eucalyptus and menthol, providing additional benefits for energy metabolism and immune support.)
Iodine - https://iherb.com/pr/life-flo-liquid-iodine-plus-natural-orange-2-fl-oz-59-ml/69888

Additional elements, where indicated by Oligoscan:

Fulvic Acid “complexed” Copper - https://goodstate.com/collections/ionic-copper/products/good-state-liquid-ionic-copper-96-servings-at-2mg-each-plus-2-mg-fulvic-acid-8-fl-oz
Fulvic Acid “complexed” Iron - https://goodstate.com/collections/ionic-iron/products/good-state-liquid-ionic-iron-48-servings-at-10mg-plus-2-mg-fulvic-acid-8-fl-oz
Sulphur - https://www.naturitas.com/#/dffullscreen/query=msm%20%20salud
Vanadium - https://www.naturitas.com/#/dffullscreen/query=ifigen%20vanadium
Cobalt - https://www.naturitas.com/#/dffullscreen/query=ifigen%20cobalt
Bismuth - https://www.naturitas.com/#/dffullscreen/query=ifigen%20bismuth
Germanium GE-132 - https://www.nutricology.com/organo-germanium-50-vegetarian-capsules

Foods, salts (use local sources):

Sodium Chloride  - https://www.amazon.com/Amazon-Brand-Happy-Ground-Ounces/dp/B07QW1G8MW/
Potassium Chloride - https://www.amazon.com/No-Salt-Substitute-11/dp/B00KPX1YPM/
Ginger (ground) - https://www.amazon.com/Organic-Ground-Anthonys-Gluten-Free-Non-GMO/dp/B019ZW9XBA/
Oat Bran - https://www.amazon.com/Bundle-Two-16oz-Packages-BELLATAVO-Certified/dp/B09MCPY86H

DIY Antiseptic Recipe (or use local sources):

Tea Tree Oil - https://iherb.com/pr/now-foods-organic-essential-oils-tea-tree-1-fl-oz-30-ml/956
Clove Oil - https://iherb.com/pr/now-foods-essential-oils-clove-2-fl-oz-59-ml/21119
MCT Oil - https://iherb.com/pr/now-foods-sports-mct-oil-unflavored-16-fl-oz-473-ml/72853
NAC Powder - https://iherb.com/pr/now-foods-nac-pure-powder-4-oz-113-g/114824
Empty Bottles -  https://www.amazon.com/PrettyCare-Measured-Pipettes-Tincture-Essential/dp/B07RK5SFYH/



[EU / Europe - alternate / domestic sources for some products]
Standard supplements, regardless of HTMA:

NAC - https://www.amazon.de/dp/B07X58JBY8
Reishi - https://www.lifeextensioneurope.de/reishi-extract-mushroom-complex
Lions Mane - https://www.amazon.de/dp/B07W668CZF
PQQ + CoQ10 - https://www.sunday.de/en/polyphenol-21-komplex.html

Additional elements, as needed:

Calcium + Phosphorus - https://www.sunday.de/en/natural-calcium-400mg-high-dose-capsules-set.html
Potassium - https://www.amazon.de/-/en/Potassium-Tablets-Vegan-Additives-Dosage/dp/B08VRPR39L/

Foods, salts:

Sodium Chloride - https://www.amazon.de/-/en/dp/B07PBJG722
Potassium Chloride - https://www.amazon.de/-/en/No-Salt-Sodium-Free-Alternative/dp/B000H185N6/
Oat Bran - https://www.amazon.de/-/en/764087/dp/B06WVLBX5P
Ginger - https://www.amazon.de/Ginger-Powder-120-Additives-Laboratory/dp/B09B2GRNG7

DIY Antiseptic Recipe:

Tea Tree Oil - https://www.amazon.de/Pure-Organic-Tea-Tree-50ml/dp/B072LZFK3Y
Clove Oil - https://www.amazon.de/Cosmetics-Organic-Carnation-Essential-Oil/dp/B0153Q871E
MCT Oil - https://www.amazon.de/dp/B0BGYFLD58
NAC Powder - https://www.amazon.de/N-Acetyl-L-Cysteine-Powder-Sulphur-Containing-Fermentation-Additives/dp/B07D3722WS
Empty Bottles - https://www.amazon.de/HandsUnity-Pipette-Bottle-Small-Glass/dp/B094DFK7F5/


[AU / Australia - alternate / domestic sources for some products]
Standard supplements, regardless of HTMA:

PQQ + CoQ10 - https://return2health.com.au/jarrow-qh-pqq-ubiquinol-pyrroloquinoline-quinone
Vimergy B12 - https://floweroflife.com.au/shop/vimergy-b12/
Psyllium Husk - https://www.woolworths.com.au/shop/productdetails/479953/macro-organic-psyllium-husk

Additional elements, as needed:

Magnesium (transdermal. NB the iHerb product above is superior) - https://www.chemistwarehouse.com.au/buy/84838/bioglan-active-magnesium-cream-100g

Foods, salts:

Sodium Chloride - https://www.woolworths.com.au/shop/productdetails/320992/mckenzie-s-natural-salt-sea
Potassium Chloride - https://ozdingo.com.au/products/pure-potassium-chloride-powder
Oat Bran - https://www.woolworths.com.au/shop/productdetails/203867/woolworths-oat-bran
Gingerhttps://www.woolworths.com.au/shop/productdetails/680510/woolworths-crushed-ginger

DIY Antiseptic Recipe:

Tea Tree Oil - https://www.chemistwarehouse.com.au/buy/86222/thursday-plantation-tea-tree-oil-100ml
Clove Oil - https://www.chemistwarehouse.com.au/buy/85247/thursday-plantation-clove-oil-13ml
MCT Oil - https://www.woolworths.com.au/shop/shopping/937713-9349580000651
NAC Powder - https://www.ebay.com.au/itm/223966754293
Empty Bottles - https://www.amazon.com.au/Magic-Season-Boston-Bottles-Dropper/dp/B09919MRFD/

[Removed / replaced in this update]
NAD+, Berberine, Vitamin A, Taurine, TTFD, Vitamin C + D-Ribose


[Supplements currently in testing for future updates, known benefits]
Rosemary Extract - glucose uptake, catecholamines, ACh, muscle preservation, anti-inflammatory, increases catecholamines
Fulvic “Trace Elements” - potential replacement for the additional elemental supplements


STEP 3 - CALCULATE SUPPLEMENTS / DOSES

Except for electrolytes which have set daily targets, adjust / remove / replace mineral supplements using your Oligoscan (and HTMA report) data as an approximate guide.

It is generally expected that ACTUAL deficiencies for magnesium, silicon, iodine, selenium, molybdenum and others may show in your Oligoscan data and that FUNCTIONAL deficiencies for iron, manganese and copper  may exist, along with various others. These may take some months to restore. Recheck your Oligoscan for progress on remineralisation (and HTMA) as desired for progress on removing sources of inflammation. Adjust supplements as needed. At 6 weeks, HTMA averaged results will approximate 3 weeks progress, assuming linear progress.

If your HTMA and/or Oligoscan profile shows elevated levels of heavy metals (eg. lead, mercury or uranium), taking a final binder (eg. 5g of activated charcoal, micronised zeolite or bentonite clay) or 5g of Pectasol-C could be appropriate before bed and far away from meals. The included spirulina already has functions here. Join us online for help.

Where a HTMA profile shows low potassium and similarly low rubidium, the rubidium deficiency will need correcting or else potassium levels may be difficult to restore. Similarly calcium with strontium and magnesium with lithium. High zinc can indicate low histidine / insufficient protein.

NB. Consuming higher than recommended daily intakes of minerals where a deficiency is not present could create additional health problems and should be generally avoided. Consuming certain minerals on an empty stomach will quite likely induce nausea.


DIET AND ELECTROLYTES

Foods to generally consume as part of this protocol, per HTMA results:
Brazil and other nuts (silicon, rubidium, strontium, cobalt, warning - high selenium)
Liver, organ meats (very nutrient dense, not every day)
Broccoli + radish / mustard seed (additional sulforaphane, trace elements)
Cabbage (zirconium, rubidium), Carrots (Vitamin A), Green beans (silicon),
Eggs (Iron, iodine, choline, lecithin, biotin, etc), Pumpkin seeds/oil (phosphorus),
Beetroot (nitrates for blood volume, nitric oxide and dopamine synthesis),
Ginger 2.5-5g/day (gut microbiome dysbiosis and itaconate / isocitrate lyase inhibition),
Oat bran (40 g+ /day for beta glucans, silicon - immunity, itaconate pathway),
Pineapple (100-200 g for silicon, bromelain - fibrin clots). Generally avoid fructose.
High protein diet (essential amino acids, often good phosphate sources) >1.5g/kg/day
Low-GI carbs 1.5-2g/kg/day, unless employing a ketogenic diet.

Hydration:
2.5-3L of water / day to help prevent dehydration and red blood cell rouleaux formation. 

Other electrolytes (potassium, sodium, calcium, magnesium, phosphate, bicarbonate):
Maintaining electrolytes can be challenging, due to expected renal dysfunction. Electrolytes are required for ion channels / transporters and signalling pathways. Where deficient, neurological symptoms, muscle spasms and pain/inflammation may occur. Nutrient absorption may be impaired. Electrolytes should be consumed slowly over the day and typically with carbohydrates / food. Consult your doctor, if suffering from any pre-existing kidney disease.
Total daily (elemental) targets -
Sodium: 2-2.5 g, eg. 5 g (teaspoon) of table salt.
Potassium: >5 g, eg. 900g / 3 large potatoes, or eg.10g of potassium chloride (Nu-Salt).
Magnesium: >750 mg. 1.5-2 g, where deficient.
Calcium: >750 mg. 1.5-2 g, where deficient
Phosphate: 1 g for maintenance, higher where deficient. eg. Meat, dairy, pumpkin seeds, red lentils, sunflower seeds, potatoes. Supplements which combine phosphate with various electrolytes, eg. calcium phosphate may be available in some regions.

Elemental weights, by compound, for calculating servings of various electrolytes:
Sodium Chloride | 39% sodium, 61 % chloride
eg. 6.4 g = 2.5 g sodium, 3.9 g chloride
Potassium Chloride | 52% potassium, 48% chloride
eg. 9.6 g = 5 g potassium, 4.6 g chloride
Calcium Phosphate | 39% calcium, 20% phosphorus
eg. 3.8 g = 1500 mg calcium, 770 mg phosphorus


STEP 4 - START THE PROTOCOL

It is fully expected that people exploring this protocol are highly sensitive to supplements.
To help reduce expected paradoxical symptoms and herxheimer effects from any improved immune activity, a starting order has been provided. Deviations from this starting order may induce avoidable and unpleasant symptoms, however you can space out the stages further, as needed.

BEFORE YOU START

1) First assess, remediate and maintain your lymphatic system as described earlier. Take note of any tissues which have been or continue to be sore / stiff / inflamed - these will be expected to flare during immune activity. These may also be adjacent to other infected tissues.

2) Due to fulvic acid’s potent and desired ability to “liberate” and recirculate metals that are being sequestered during chronic inflammation, it is suggested that you test your response to a single drop of the the Good State Fulvic Ionic Man (multi-mineral) in a glass of water before starting weeks 5-8 of the protocol. If you experience any unpleasant effects, take a few days to titrate up from a smaller dose (eg. place a drop in a bottle of water, mix and then transfer a drop from this first dilution into another glass of water) until you can consume a standard serving without observing these effects.

3) If you have nasal inflammation or notice a bottleneck when breathing in, performing a MARCoNS test through your health practitioner could be appropriate. Candida, Aspergillus, Staphylococcus and Klebsiella species are unwanted microorganisms sometimes found in the nasopharynx. Various supplements may provide additional benefits against these pathogens, by inhibiting their energy availability, breaking down biofilms and other mechanisms.

NB. An unpleasant “herxheimer-like” effect would be expected during any increased immune response activity.

4) If your intracellular magnesium levels are low, you may experience “B6 toxicity” symptoms which would be enhanced by consuming additional B6 in any form. Gut motility issues are also commonly experienced.

You may help avoid unwanted symptoms by FIRST consuming the food items and electrolytes to reach the suggested total daily intakes for 7 - 10 days.. Liberally applying the magnesium gel 2+ times / day covering armpits to elbows, stomach, etc. can be highly effective. The gel also has eucalyptus and menthol, which has an antiseptic function.

NB. Correcting severe electrolyte deficiencies can create temporary paradoxical effects and increased rates of metabolism, heart rate, etc.

5) Where described below, “x 3” means “3 times per day” and not “3 doses all at once”.


Weeks 1-2+

Perform regular lymphatic maintenance. Performing pacing.
Start consuming / absorbing the foods and various electrolytes discussed in Step 3 & 4, plus:

Liposomal Riboflavin 100 mg, 2 x 2 small scoops. (This will reduce over time.)
Niacinamide (no-flush) > 500mg x 1-2 (can increase as needed)
PQQ 20mg, CoQ10 200 mg x 1-3
Acetyl L-Carnitine 1000 mg x 2
Vitamin D3 5000 IU - 8000 IU x 1
DHA 300-500 mg x 1
R-ALA  100 mg x 1 (AMPK promoter)
Apigenin eg. 50mg x 1 AM (p38 MAPK inhibitor and NADase inhibitor, start at ¼ dose and increase. Upper threshold for effective dose may be 400mg/day.)
Vimergy B12 x 1 serving (sublingual)
Silica x 2 servings (and/or eat cooked green beans, brazil nuts)
Anti-fungal enzymes (please read product label carefully to set expectations)
Iodine approx 500mcg (half a dropper) in water
L-Serine 2000mg
D-Ribose 5g (teaspoon)
Youtheory Spore Probiotic 2 capsules

Morning, before any food -

Psyllium Husk “shake” - a teaspoon of powder in a glass of water. (Will assist toxin removal, gut microbiome / GI motility).


Weeks 3-4+

Continue items in all previous weeks and add:

Folinic Acid (NOT Folic) 800-1200 mcg x 1
EGCG 100-200 mg x 2-3
A-GPC Choline >250 mg x 2 (If muscle stiffness or headache occurs, reduce or pause dosing.)

Slowly add 1g (1/5th tsp) - 10g (2 tsp) of Spirulina to the morning psyllium husk shake.
(May initially cause nausea, headache, fatigue from fungal die-off symptoms - start low and slow.)


Weeks 5-8+

Continue items in all previous weeks APART FROM THE IODINE and add:

R-ALA  (Increase to 100mg x 3 for 3 weeks and then reduce to 100 mg x 1.)
NAC (Thrombolytic, biofilm breaker and mineral chelator - first 3 weeks at 1000 mg x 3, then reduce to < 500 mg x 1)
Forskolin 10 mg x 1-2 (Temporary adrenergic increase, reduced histamine.)
Artichoke (Luteolin) 25 mg x 1 (Temporary adrenergic increase, reduced histamine.)
ACV 2-3 caps x 3 (may cause temporary GI upset, histamine increase.)
Life Extension Reishi Complex* x 2
*A 1-3 day initial headache is possible when starting reishi.
Lions Mane (beta glucans) >250mg x1 AM (may increase histamine / insomnia, low dose.) [..]
Thorne Basic B Complex 1 cap x 1-3 (Can expect to reduce this over time as key minerals are restored. Excess can cause peripheral neuropathy, via B6 / P5P.)

Morning, after food -
Good State Fulvic Ionic Man Multi Mineral x 2 servings (Start with lower dose.)

Lunchtime, after food  -
ALTERNATE EACH DAY, AS INDICATED BY YOUR OLIGOSCAN, EITHER
Good State Fulvic Ionic Copper x eg. 2-4 servings OR
Good State Fulvic Ionic Iron x 2-4 servings 

Evening, after food -
Good State Fulvic Ionic Man Multi Mineral x 2 servings (Start with lower dose.)
+ Vanadium, Cobalt, Bismuth, Germanium, Sulphur (where indicated)


STEP 5 - OPTIONAL FOLLOW UP

When the element levels reported by your HTMA are optimal, a 3 day “water fast” may provide additional benefits. This is NOT advisable with severe mineral deficiencies.

If this is your first water fast, it is generally advisable to have someone monitoring you during this time. Avoid driving vehicles, stress and excessive exertion.

Fasting protocol requirements (per day):
No food or other supplements.
3L water.
5g (teaspoon) of sodium chloride (table salt = 2.5g sodium elemental)
1g of baking soda (110 mg of sodium)

(Optional add-ons)
500mg EGCG x 3*
Resveratrol 120mg x 3
Hesperidin 500mg x 2


(*NB. Important safety consideration: To break a water fast “early”, use 2 or more Apple Cider Vinegar doses - without capsules - as a succinate source.
This is only a requirement if EGCG has been dosed in the previous 6 hours.

Failure to observe this safety consideration may result in rapid cyclical blackouts and convulsions until the EGCG is fully metabolised or until someone else administers Apple Cider Vinegar.
This could easily be fatal if eg. operating a motor vehicle. YOU HAVE BEEN WARNED.)

« Last Edit: February 04, 2023, 07:32:32 PM by joshua.leisk »
NB. I am NOT a doctor and all information provided is for educational purposes only.

Please consult your physician before attempting anything you read here.