Research Group for ME/CFS, Chronic Disease, Ageing and Cancer

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v3.48U (preview)
« on: April 09, 2022, 08:43:52 PM »
General Mitochondrial and Wellness Protocol
(Author: Joshua Leisk, V3.48U - 4th August 22)

This is a dietary supplement based protocol which may be helpful for supporting the unique nutritional requirements of people with:

Myalgic Encephalomyelitis / Chronic Fatigue Syndrome (ME/CFS).
Long COVID / Long Haulers.
Viral / Post-Viral Chronic Fatigue.
Breathlessness, Hypoxia.
Acidosis, Alkalosis / pH Dysregulation.
Dysregulated Cortisol, Dyslipidemia, Alopecia.
Small Fibre Peripheral Neuropathy (SFPN).
Mast Cell Activation Syndrome (MCAS).
Diamine Oxidase (DAO) Deficiency and Elevated Histamine.
Brain Fog.
Major and other types of Depression.
Postural Orthostatic Tachycardia Syndrome (POTS).
Chronic Inflammatory Response Syndrome (CIRS), Fibromyalgia.
Frequent Urination.
GI Disorders, Small Intestinal Bowel Overgrowth (SIBO).
B6 Toxicity.
Ehlers Danlos Syndrome (EDS), Joint Pain, Connective Tissue Disorders.
Polycystic Ovary Syndrome (PCOS).
Erectile Dysfunction.
Elevated Cholesterols / Fasting Glucose / D-Dimer.

NB. While all efforts have been made to eliminate them, some temporary paradoxical effects may be observed during commencement. As deficiencies are corrected and neurotransmitter homeostasis is reset, paradoxical effects may include: temporary adrenaline and heart rate increases. Existing "burning" sensations and headache may also flare. Temporary alterations to libido may be expected.

Monitoring Lactate Dehydrogenase (LDH) - target 100-150 mmol/L, d-dimer, cholesterols and fasting glucose via blood tests would be suggested.

Disclaimer: Always consult with your doctor, especially if taking medications, eg. SNRI /SSRI, beta blockers, MAO inhibitors, etc.


A baseline Hair Toxin Mineral Analysis (HTMA) is required to personalise mineral supplementation. For this purpose, a list of vendors offering a compatible list of markers and reference ranges is provided here, by laboratory, region:

Online resellers for Trace Elements Inc:
AU - (choose Profile 1)
DE -
FR -
NZ -
PL -
SG -
UK -
US & CAN -

Other compatible laboratories:
AU -
EU -
EU - (doesn't include rubidium)
EU - (doesn't include rubidium)
NZ -
UK -
US - (Not available in NY/ NYC and other areas.)

Hair collection guide:
1. Please ignore any conflicting vendor-specific instructions.
2. The hair needs to be clean, well-rinsed, dry, untreated and uncoloured. Unwashed hair may provide false (elevated) data for sodium and potassium, from dried sweat.
3. Select suitable areas of either scalp, neck, pubic or beard hair. If the hair is longer than 2.5cm / 1", first trim the sample area to this maximum length with clean stainless steel scissors. By using shorter hair, the report data relates to a more recent period of time.
4. Cut / harvest the (remaining) hair to be sampled as close to the skin as possible. Do not use clippers or an electric razor. Collect at least 2 heaped tablespoons worth of hair, or approximately 250 mg. If the amount of hair provided is insufficient, the sample may be rejected.
5. Place the hair sample in the sample kit envelope provided, or in a clean, clearly labelled envelope or sachet. Do not use ziplock bags as apparently hair “sweats” and the sample may be rejected.
6. Repeat every eg. 6 weeks, depending on your dosing aggressiveness and using fresh regrowth from the same area, until optimal mineral balance is achieved.


It will typically take 2-3 weeks to get your HTMA results and a potentially similar timeframe to order / receive supplements from any overseas vendors. Now would be an opportunistic time to order any/all supplements which do not require personalisation by HTMA results and presumptively, at least the 3 mineral supplements which are used early in the schedule. Expect 3+ months to remineralise from severe mineral deficiencies.

Some products are not available domestically in all regions and will need to be imported from the US. As such, the “master list” is based around US suppliers. However, a list of local vendors / supplements for regions has been provided for some products. It is expected that local lists will expand over time.

[USA / Master List - most products ship internationally]
Standard supplements, regardless of HTMA:
Liposomal Riboflavin* -
(*This is a unique product - do not substitute. Yes, the international shipping price is unusually high, although if you buy 2, a cheaper shipping option appears and the total order price doesn’t change. No, we haven’t been able to find alternatives. Digestion and absorption / transport bottlenecks exist for riboflavin. Multiple alternate forms have been tested without success. This microencapsulated product bypasses these issues.)

[iHerb Quicklist for this section - ]
PQQ + CoQ10 -
Thorne Basics B Complex* -
(*Do not substitute. This B complex product has been carefully selected to contain sufficient amounts of the required forms of each coenzyme, minus inappropriate amounts of pyridoxine, yet adequate P5P.)
Folinic Acid (not folic acid) -
Vitamin D3 -
Vitamin A -
Vitamin C -
A-GPC Choline -
Forskolin -
Artichoke / Luteolin -
Apigenin -
Reishi* -
(*PRODUCT DOES NOT SHIP TO EU - use EU source. Do not substitute. A USP study found approximately 75% of reishi products are fake. This product passed our HPLC + LCMS mass spectrometer testing. Most did not. Further, different metabolites are extracted from different parts of the fungi, eg. fruiting body, spores and mycelium. Beta-glucans require hot water extracts, triterpenes require alcohol extracts.)
Lions Mane* -

Mineral supplements, order per your HTMA results:
[iHerb Quicklist for this section (remove any unneeded) - ]
Copper -
Manganese -
Iron -
Calcium Phosphate (Marine) -
Magnesium -
Lithium -
Molybdenum -
Selenium -
Vanadium -
Chromium -
Zinc -
Iodine -
Strontium -
Boron -

Additional minerals from alternate vendors:
Rubidium -
Cobalt -
Germanium GE-132 -

Other alternative products and sources:
TTFD / Allithiamine -
Liposomal Iron (useful for feeding tubes) -
Trisodium Phosphate -
Magnesium Phosphate - US
Calcium Phosphate -
Potassium Phosphate -
Liver -

Foods, Salts (use local sources):
Sodium Chloride  -
Potassium Chloride -
Ginger (ground) -
Oat Bran -

[DE / Germany - alternate / domestic sources for some products]
Standard supplements, regardless of HTMA:
Reishi -
Lions Mane -
PQQ + CoQ10 -

Mineral supplements, order per your HTMA results:
Liposomal Iron -
Copper -
Manganese -
Liposomal Magnesium -
Iodine -
Selenium -
Molybdenum -
Chromium -
Boron -
Calcium + Phosphorus -
Potassium -

Foods, salts:
Sodium Chloride -
Potassium Chloride -
Oat Bran -
Ginger -

[AU / Australia - alternate / domestic sources for some products]
Standard supplements, regardless of HTMA:
PQQ + CoQ10 -

Mineral supplements, order per your HTMA results:
Manganese -
Chromium -
Magnesium -
Iodine -

Other alternative products and sources:
Sodium Phosphate -
Disodium Phosphate -
Potassium Phosphate -
Calcium Phosphate -

Foods, salts:
Sodium Chloride -
Potassium Chloride -
Oat Bran -


Adjust / remove / replace mineral supplements using your HTMA report as a guide.

The general expectation is that deficiencies for iron, manganese (and copper, typically where lead, mercury or uranium are measured low) will exist, along with others. These may take some months to restore. Daily Values of minerals may need to be (aggressively) exceeded, relative to indicated deficiency level. Where results show that individual mineral levels are significantly lower than others, an individual mineral supplement and much higher doses could be needed. Recheck every 4-6 weeks. At 6 weeks, averaged results will approximate 3 weeks progress.

If your HTMA profile shows elevated levels of heavy metals (eg. lead, mercury or uranium), taking a final binder (eg. 5g of activated charcoal, micronised zeolite or bentonite clay) could be appropriate before bed and far away from meals.

Where a HTMA profile shows low potassium and similarly low rubidium, the rubidium deficiency will need correcting or else potassium levels may be difficult to restore.

NB. Consuming higher than recommended daily intakes of minerals may be required where a deficiency is present, however doing so where a deficiency is not present could create additional health problems and should be generally avoided. Consuming minerals on an empty stomach is likely to induce nausea.

Foods to generally consume as part of this protocol, per HTMA results:
Brazil and other nuts (rubidium, strontium, cobalt, warning - high selenium)
Liver (very nutrient dense - supplemental options also provided)
Broccoli + radish / mustard seed (additional sulforaphane, trace elements)
Cabbage (zirconium, rubidium)
Carrots (Vitamin A)
Eggs (Iron, iodine, choline, lecithin, biotin, etc)
Beetroot (nitrates for blood volume, nitric oxide and dopamine synthesis)
Ginger - 2.5-5g/day (gut microbiome dysbiosis and itaconate / isocitrate lyase inhibition)
High protein diet (essential amino acids, often good phosphate sources)
Oat bran (40g+ /day for beta glucans - immunity, itaconate pathway)

2.5-3L of water / day to help prevent dehydration and red blood cell rouleaux formation. 

Other electrolytes (potassium, sodium, calcium, magnesium, phosphate, bicarbonate):
Maintaining electrolytes can be challenging, due to expected renal dysfunction. Electrolytes are required for ion channels / transporters and signalling pathways. Where deficient, neurological symptoms, muscle spasms and pain/inflammation may occur. Nutrient absorption may be impaired. Electrolytes should be consumed slowly over the day and typically with carbohydrates / food. Consult your doctor, if suffering from any pre-existing kidney disease.
Total daily (elemental) targets -
Sodium: 2-2.5g, eg. 5g (teaspoon) of table salt.
Potassium: >5g, - eg. 900g / 3 large potatoes, or eg. 10g of potassium chloride (NoSalt / Nu-Salt).
Magnesium: >750mg. 1.5-2g, where deficient.
Calcium: >750mg. 1.5-2g, where deficient
Phosphate: 1 g for maintenance, higher where deficient. eg. Meat, dairy, red lentils, sunflower seeds, potatoes. Supplements which combine phosphate with various electrolytes, eg. calcium phosphate may be available in some regions.

Elemental weights, by compound, for calculating servings of various electrolytes:
Sodium Phosphate Dibasic | 32% sodium, 22% phosphorus
eg. 7.8g = 2.5g sodium, 1.7g phosphorus
Trisodium Phosphate | 42% sodium, 19% phosphorus
eg. 6g = 2.5g sodium, 1.1g phosphorus
Sodium Chloride | 39% sodium, 61 % chloride
eg. 6.4g = 2.5g sodium, 3.9g chloride
Sodium Bicarbonate (Baking Soda) | 27% sodium (best not consumed near meals)
eg. 9.3g = 2.5g sodium + bicarbonate overdose (diarrhoea, in a single dose)
Potassium Phosphate | 55% potassium, 15% phosphorus
eg. 9g = 5g potassium, 1.4g phosphorus
Potassium Chloride | 52% potassium, 48% chloride
eg. 9.6g = 5g potassium, 4.6g chloride
Magnesium Monophosphate | 28% magnesium, 23% phosphorus
eg. 2.7g = 750 mg magnesium, 600 mg phosphorus
Calcium Phosphate | 39% calcium, 20% phosphorus
eg. 3.8g = 1500 mg calcium, 770 mg phosphorus


It is fully expected that people exploring this protocol are highly sensitive to supplements.
To help avoid paradoxical symptoms, a starting order has been provided. Deviations from this starting order may induce avoidable and unpleasant symptoms.

[NB. Dosing schedule - where indicated, eg. “x 3” means 3 times per day, not 3 doses all at once.]

Days 1-3+
Start consuming the foods and various electrolytes discussed in section 3, plus:
Liposomal Riboflavin 200-250 mg, 2 x 2 large scoops, or 1 large scoop, x4. (This will reduce over time, as manganese / copper / selenium levels increase.)
PQQ 20mg, CoQ10 200 mg x 1-3
Vitamin D3 5000 IU - 8000 IU x 1
Vitamin A 3000 mcg x 1 (or eat carrots)
DHA 300-500 mg x 1
SOD 250mg x 1 (new in v3.47)

Morning, with food (only where indicated by your HTMA results) -
Copper | eg. 5-10 mg (May cause insomnia with low riboflavin or very low iron.)
Manganese | eg. 20-40 mg (Very well-tolerated, unless suffering from Lyme Disease.)
Selenium | eg. 400-800 mcg

Days 4-6+

Continue taking the items in all previous days and add:
Thorne Basics B Complex 1 cap x 1-3 (Can reduce over time)
Folinic Acid (NOT Folic) 800-1200 mcg x 1
Vitamin C 500 mg x 1-2
EGCG 100-200 mg x 2-3
A-GPC Choline >250 mg x 2 (If muscle stiffness or headache occurs, reduce or pause dosing.)

Days 7+

Continue taking the items in all previous days and add:
Forskolin 10 mg x 1-2 (Temporary adrenergic increase, reduced histamine.)
Artichoke (Luteolin) 25 mg x 1 (Temporary adrenergic increase, reduced histamine.)
R-ALA 150-200 mg x 3
NAC (First 3 weeks at 1000 mg x 3, then reduce to 500 mg x 1.)
TTFD 50mg x 1 (Increase dose from 1/10th capsule, temporary neuro symptoms expected.)
ACV 2-3 caps x 3 (may cause temporary GI upset, histamine increase.)

Evening, with food (all other minerals, where indicated by your HTMA results) -
Zinc, Iodine, Molybdenum, Boron, Chromium, Vanadium, Germanium, Lithium, Rubidium, Cobalt (introduce slowly, if needed)

Days 10+

Continue taking the items in all previous days and add:
Life Extension Reishi Complex* x 2
*A 1-3 day initial headache is possible when starting reishi.
Lions Mane (beta glucans) >250mg x1 AM (may increase histamine / insomnia, low dose.)
Apigenin eg. 50mg x 1 AM (p38 MAPK inhibitor and NADase inhibitor, start at ¼ dose and increase. Upper threshold for effective dose may be 400mg/day.)

Iron eg. 25mg x 3-4 (Sublingual or liposomal dosing only)
With "anaemia of inflammation", iron absorption is blocked in the GI tract. Sublingual or liposomal forms are needed. An IV iron infusion may be appropriate, where indicated by your healthcare professional. Any rapid ferritin increase can cause or accelerate hypophosphatemia. Care should be taken to consume optimal levels of phosphates, especially where already deficient. If an active eg. bacterial infection is present, that should be resolved before starting iron supplementation.


When the element levels reported by your HTMA are optimal, a 3 day “water fast” may provide additional benefits. This is NOT advisable with severe mineral deficiencies.

If this is your first water fast, it is generally advisable to have someone monitoring you during this time. Avoid driving vehicles, stress and excessive exertion.

Fasting protocol requirements (per day):
No food or other supplements.
3L water.
5g (teaspoon) of sodium chloride (table salt = 2.5g sodium elemental)
1g of baking soda (110mg of sodium)

(Optional add-ons)
500mg EGCG x 3*
Resveratrol 120mg x 3
Hesperidin 500mg x 2

(*NB. Important safety consideration: To break a water fast “early”, use 2 or more Apple Cider Vinegar doses - without capsules - as a succinate source.
This is only a requirement if EGCG has been dosed in the previous 6 hours.

Failure to observe this safety consideration may result in rapid cyclical blackouts and convulsions until the EGCG is fully metabolised or until someone else administers Apple Cider Vinegar.
This could easily be fatal if eg. operating a motor vehicle. YOU HAVE BEEN WARNED.)

[A downloadable PDF is available with free registration or via our Discord server.]

« Last Edit: August 06, 2022, 04:42:45 AM by joshua.leisk »
NB. I am NOT a doctor and all information provided is for educational purposes only.

Please consult your physician before attempting anything you read here.